Recombinant Saccharomyces cerevisiae tRNA 2'-phosphotransferase (TPT1)

中文名稱：

釀酒酵母TPT1重組蛋白
貨號(hào)：

CSB-YP614198SVG
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

釀酒酵母TPT1重組蛋白
貨號(hào)：

CSB-EP614198SVG
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

釀酒酵母TPT1重組蛋白
貨號(hào)：

CSB-EP614198SVG-B
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

釀酒酵母TPT1重組蛋白
貨號(hào)：

CSB-BP614198SVG
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

釀酒酵母TPT1重組蛋白
貨號(hào)：

CSB-MP614198SVG
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

TPT1
Uniprot No.：

Q12272
別名：

TPT1; YOL102C; HRE230; tRNA 2'-phosphotransferase; EC 2.7.1.160
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-230
氨基酸序列

MRQVLQKDKR DVQLSKALSY LLRHTAVKEK LTIDSNGYTP LKELLSHNRL KTHKCTVDDI HRIVKENDKQ RFHIKTLGAD EEWICATQGH SIKSIQPSDE VLVPITEASQ LPQELIHGTN LQSVIKIIES GAISPMSRNH VHLSPGMLHA KGVISGMRSS SNVYIFIDCH SPLFFQTLKM FRSLNNVYLS SSIPVELIQK VVVKGNLKDE EKLDTLRRIL HERNIPLEKI
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.
基因功能參考文獻(xiàn)：
1. examined 21 Tpt1 protein variants mutated in conserved residues or blocks of residues and show that one of them, Tpt1 K69A/R71S protein, accumulates large amounts of intermediate with trinucleotide substrate due to a very slow second step PMID: 15611300
2. identified four residues-Arg23, His24, Arg71, and Arg138-as essential for Tpt1 function in vivo PMID: 15611301
蛋白家族：

KptA/TPT1 family
數(shù)據(jù)庫(kù)鏈接：

KEGG: sce:YOL102C

STRING: 4932.YOL102C