Recombinant Mouse E3 ubiquitin-protein ligase RAD18 (Rad18)

中文名稱：

小鼠Rad18重組蛋白
貨號：

CSB-YP886165MO
規(guī)格：
來源：

Yeast
其他：

中文名稱：

小鼠Rad18重組蛋白
貨號：

CSB-EP886165MO
規(guī)格：
來源：

E.coli
其他：

中文名稱：

小鼠Rad18重組蛋白
貨號：

CSB-EP886165MO-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Rad18重組蛋白
貨號：

CSB-BP886165MO
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Rad18重組蛋白
貨號：

CSB-MP886165MO
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Rad18
Uniprot No.：

Q9QXK2
別名：

Rad18; Rad18sc; E3 ubiquitin-protein ligase RAD18; EC 2.3.2.27; Postreplication repair protein RAD18; mRAD18Sc; RING-type E3 ubiquitin transferase RAD18
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-509
氨基酸序列

MEVLAEPRWP PGLAVMKTID DLLRCGICFE YFNIAVIIPQ CSHNYCSLCI RKFLSYKTQC PTCCVAVTEP DLRNNRLLDE LVKSMNFART HLLQFALESP PISPVSSTSK KVVVKVHNAD AAQHPVKQAN RLMDKFLIRE TGDCVFELLG KENERKFSPQ KELSTSAEIK ETSLLGKPVL GLSDANGPVT PSTSTMKLDT KVSCPVCGVS IPENHINKHL DSCLSREEKK ESLRSSAHKR KPLPKTVYNL LSDRDLKKKL KQYGLSVQGN KQQLIKRHQE FVHMYNAQCD ALHPKSAAEI VQEIESMEKT RMRLEASKLN ENVMVFTKNQ TEKEIEEVHS EYRKKHQNAF QLLVDQAKKG YKKTGRVSQA AAMRTDEPAE TLPSMRTDEP AETLPSMRTD EPAETLPLMR ADEPAETLPS ECIAQEDNVS FSDTVSVTNH FPQPQLDSPG PSEPERPDDS SSCTDILFSS DSDSCNRNDQ NREVSPQQTR RTRASECVEI EPRNKRNKN
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
基因功能參考文獻(xiàn)：
1. Somatic hypermutation of immunoglobulin genes (SHM) in Rad18 KO mice was similar to wild-type mice, suggesting that Rad18 is dispensable for SHM. PMID: 28082021
2. Rad18 confers DNA damage tolerance and tumor-suppression in a physiological setting. PMID: 26883629
3. RAD18 can function as a mediator for DNA damage response signals to activate the G2/M checkpoint in order to maintain genome integrity and cell survival after IR exposure. PMID: 25675240
4. repairs meiotic double-strand DNA breaks and and maintains meiotic sex chromosome inactivation PMID: 21807948
5. RAD18 contributes to maintenance of genomic stability through postreplication repair PMID: 12509447
6. Rad18Sc localizes to the XY body and to other chromosomal regions that are unpaired and transcriptionally silenced during male meiotic prophase PMID: 15383616
7. Rad18 suppresses viral infection in a non-specific fashion, probably by targeting incoming DNA. PMID: 16710452
8. RAD18 is intrinsically required for the long-term maintenance of spermatogenesis. PMID: 19068231
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亞細(xì)胞定位：

Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
蛋白家族：

RAD18 family
組織特異性：

Expressed in thymus, spleen, brain, and ovary.
數(shù)據(jù)庫鏈接：

KEGG: mmu:58186

UniGene: Mm.103812