Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(ng/ml)
12.563
12.652
12.759
12.706
12.546
12.457
SD
0.037
0.035
0.043
0.058
0.054
0.060
CV(%)
3.978
3.743
4.570
6.183
5.813
6.494
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:20
Average %
103
Range %
94-107
1:40
Average %
96
Range %
85-100
1:80
Average %
91
Range %
85-95
1:160
Average %
91
Range %
82-94
回收率:
The recovery of mouse OSF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range%
Serum (n=5)
92
84-96
EDTA plasma (n=4)
90
83-95
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Induces cell attachment and spreading and plays a role in cell adhesion. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX.
基因功能參考文獻:
Inhibition of periostin could be important for suppressing capsule formation on silicone implants after in vivo implantation. PMID: 29854742
Unaffected by Postn, Vav-Itgav(-/-) haematopoietic stem cell proliferate faster in vitro, illustrating the importance of Postn-Itgav interaction. PMID: 27905395
mesenchymal source, specific to cardiovascular tissues, roles in cardiovascular development and disease [review] PMID: 29101484
Results indicate that periostin is a key player in the development of bleomycin-induced fibrosis and consequent enhancement of tumor cell colonization in the lung. PMID: 29498146
these data indicate that CatK not only plays a major role in bone remodeling but also modulates modeling-based cortical bone formation by degrading periostin and thereby moderating Wnt-beta-catenin signaling. PMID: 28322464
Ablation of periostin suppresses post-infarction myocardial regeneration by inhibiting the PI3K/GSK3beta/cyclin D1 signalling pathway, indicating that periostin is essential for myocardial regeneration. PMID: 28453729
this study shows that periostin regulates fibrocyte function to promote myofibroblast differentiation and lung fibrosis PMID: 27435108
Genetic or pharmaco-genetic inhibition of periostin in animal models of renal disease was efficient in arresting the progression of the kidney pathology. (Review) PMID: 28884334
TGFBI and periostin act similarly in the heart in affecting fibrosis and disease responsiveness. PMID: 28750100
Results suggest that stromal cell periostin (POSTN) supports both normal hematopoietic progenitor cells (HPCs) and leukemia-initiating cells in vitro. PMID: 27596966
the expressions of the periostin splice variants were increased in ischemic retinas. PMID: 27744020
POSTN regulates the scar formation after spinal cord injuries. PMID: 28082119
periostin in the extracellular matrix architecture prevents pulmonary leakage of blood fluid, thus increasing the survival rate in mice with acute lung injury PMID: 27270966
These results indicated that tensile stress load increased the expression levels of PN in mouse peridontal ligaments and human periodontal ligament cells. PMID: 28260019
POSTN promotes the pulmonary accumulation of myeloid-derived suppressor cells (MDSCs) during the early stage of breast tumour metastasis. Postn deletion decreases neutrophil and monocytic cell populations in the bone marrow of mice and suppresses the accumulation of MDSCs to premetastatic sites. PMID: 27193093
Fibrillin-2 and periostin are target genes in Osterix-mediated osteoblast differentiation. PMID: 27751812
Periostin mediates intestinal inflammation through the activation of NF-kappaB signaling, which suggests that periostin is a potential therapeutic target for inflammatory bowel disease. PMID: 26890265
Thus, these results demonstrate a subcellular role of periostin in promotion of fibronectin secretion from the ER. PMID: 26820539
These data demonstrate that the expression of periostin is stimulated by ErbB ligand NRG1 and influences the migration of Schwann cell precursors. PMID: 26187852
Our data suggest that POSTN is a crucial factor for proper exocrine lineage-specific regeneration after severe acute pancreatitis. PMID: 26632158
Periostin functions as a regulator of atherosclerotic lesion formation and progression. PMID: 26564821
HMGB1 expression in the periodontal ligament is differentially regulated during orthodontic tooth movement in wild type versus periostin-deficient mice. PMID: 25948513
POSTN was expressed in the region surrounding melanoma cell nests in metastatic lesions of both wounded mice and the patient. PMID: 26083413
POSTN significantly enhances neural stem cell proliferation and differentiation after hypoxia-ischemia. PMID: 25894199
loss of Postn in an ErbB2/Neu/HER2 overexpression model results in apocrine-like tumors that activate an AR-dependent pathway. PMID: 25592291
Periostin appears to be a mediator for epidermal hyperplasia that is common to AD and psoriasis. PMID: 25572557
Deficiency in periostin abrogated the development of liver fibrosis in mice. PMID: 25541330
key pathologic factors responsible for bone and periodontal ligament damage in periostin-null mice PMID: 25757567
These results further indicate that periostin is an essential mediator of cortical bone response to mechanical forces (loading and unloading). PMID: 25445447
This study found that the secreted protein periostin (Postn) is highly induced after partial pancreatectomy in regenerating areas containing mesenchymal stroma and tubular complexes. PMID: 25485969
Periostin might be associated with pathophysiology in post-ischemic inflammation and neurogenesis. PMID: 24646642
Overexpression of periostin resulted in reduced expression of peroxisome proliferator-activated receptor alpha (PPARalpha), a master regulator of fatty acid oxidation, and activation of the JNK signaling pathway. PMID: 25003192
In mice, periostin is required for maximal airways hyperresponsiveness and inflammation after house dust mite sensitization and challenge. PMID: 24996263
periostin deficiency may not only cause periodontal ligament collagen fibril disorganization, but could also affect the distribution of other major extracellular matrix proteins such as fibronectin and tenascin C. PMID: 24352874
periostin plays an essential role in the function of collagenolytic enzymes like cathepsin K, MMP1 and MMP2 in the compressed PDL after orthodontic force application. PMID: 24202437
adversely modifies the progression of renal cystic disease by promoting cyst epithelial cell proliferation, cyst enlargement, and interstitial fibrosis PMID: 24284511
Periostin-induced epithelial migration and proliferation requires mTOR signaling. PMID: 24349533
Periostin and a paracrine loop composed of IL-1alpha and IL-6 regulates keratinocyte proliferation and differentiation in the epithelial-mesenchymal interactions, and periostin tunes the magnitude of keratinocyte proliferation and differentiation. PMID: 24352037
the level of periostin expression could influence the propensity to fatigue fractures. PMID: 24167618
Periostin downregulation is an early marker of inhibited neonatal murine lung alveolar septation. PMID: 23723163
Metabolic activity of CYP1B1 contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of Postn expression. PMID: 23979599
Clear cell renal cell carcinoma induces fibroblast-mediated accumulation of stromal periostin, enhancing tumor cell attachment and fibroblast proliferation. PMID: 23896380
periostin exacerbates the pathogenesis of AD through TSLP production from keratinocytes. PMID: 22918211
Periostin plays an essential role in the pathogenesis of Bleomycin-induced scleroderma in mice. PMID: 22911870
These results suggest that periostin, which is secreted by the intraocular macrophages, enhances the hyaloidvascular system regression by intensifying the adhesion of macrophages to hyaloid vessels. PMID: 22930727
Periostin regulates beta catenin signaling and parathyroid hormone anabolic effects in bone. PMID: 22927401
Periostin overexpression is associated with kidney injury. PMID: 22167593
periostin sets up a vicious circle that links Th2-type immune responses to keratinocyte activation and plays a critical role in the amplification and chronicity of allergic skin inflammation. PMID: 22684102