Western Blot
Positive WB detected in: K562 whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), Mouse Kidney tissue lysate(30μg)
All lanes: SMARCAL1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 120s

IHC image of CSB-PA885774LA01HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody