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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus weihenstephanensis Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP435910BON
CSB-EP435910BON
CSB-BP435910BON
CSB-MP435910BON
CSB-EP435910BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP435911BON
CSB-EP435911BON
CSB-BP435911BON
CSB-MP435911BON
CSB-EP435911BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L11 (rplK) CSB-YP435912BON
CSB-EP435912BON
CSB-BP435912BON
CSB-MP435912BON
CSB-EP435912BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Methionine--tRNA ligase (metG), partial CSB-YP435913BON
CSB-EP435913BON
CSB-BP435913BON
CSB-MP435913BON
CSB-EP435913BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L3 (rplC) CSB-YP435914BON
CSB-EP435914BON
CSB-BP435914BON
CSB-MP435914BON
CSB-EP435914BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L16 (rplP) CSB-YP435915BON
CSB-EP435915BON
CSB-BP435915BON
CSB-MP435915BON
CSB-EP435915BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L30 (rpmD) CSB-YP435916BON
CSB-EP435916BON
CSB-BP435916BON
CSB-MP435916BON
CSB-EP435916BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 30S ribosomal protein S11 (rpsK) CSB-YP435917BON
CSB-EP435917BON
CSB-BP435917BON
CSB-MP435917BON
CSB-EP435917BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Phosphoglucosamine mutase (glmM) CSB-YP435918BON
CSB-EP435918BON
CSB-BP435918BON
CSB-MP435918BON
CSB-EP435918BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Homoserine kinase (thrB) CSB-YP435919BON
CSB-EP435919BON
CSB-BP435919BON
CSB-MP435919BON
CSB-EP435919BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP435920BON
CSB-EP435920BON
CSB-BP435920BON
CSB-MP435920BON
CSB-EP435920BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP435921BON
CSB-EP435921BON
CSB-BP435921BON
CSB-MP435921BON
CSB-EP435921BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP435922BON
CSB-EP435922BON
CSB-BP435922BON
CSB-MP435922BON
CSB-EP435922BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Ribonuclease Y (rny), partial CSB-YP435923BON
CSB-EP435923BON
CSB-BP435923BON
CSB-MP435923BON
CSB-EP435923BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP435924BON
CSB-EP435924BON
CSB-BP435924BON
CSB-MP435924BON
CSB-EP435924BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Regulatory protein recX (recX) CSB-YP435925BON
CSB-EP435925BON
CSB-BP435925BON
CSB-MP435925BON
CSB-EP435925BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP435926BON
CSB-EP435926BON
CSB-BP435926BON
CSB-MP435926BON
CSB-EP435926BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP435927BON
CSB-EP435927BON
CSB-BP435927BON
CSB-MP435927BON
CSB-EP435927BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP435928BON
CSB-EP435928BON
CSB-BP435928BON
CSB-MP435928BON
CSB-EP435928BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP435929BON
CSB-EP435929BON
CSB-BP435929BON
CSB-MP435929BON
CSB-EP435929BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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