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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus weihenstephanensis Methylmalonate semialdehyde dehydrogenase [acylating] 1 (iolA1) CSB-YP431508BON
CSB-EP431508BON
CSB-BP431508BON
CSB-MP431508BON
CSB-EP431508BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Methylthioribulose-1-phosphate dehydratase (mtnB) CSB-YP431509BON
CSB-EP431509BON
CSB-BP431509BON
CSB-MP431509BON
CSB-EP431509BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP431510BON
CSB-EP431510BON
CSB-BP431510BON
CSB-MP431510BON
CSB-EP431510BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Putative 8-amino-7-oxononanoate synthase (bioF) CSB-YP431511BON
CSB-EP431511BON
CSB-BP431511BON
CSB-MP431511BON
CSB-EP431511BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Probable butyrate kinase (buk) CSB-YP431512BON
CSB-EP431512BON
CSB-BP431512BON
CSB-MP431512BON
CSB-EP431512BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Putative metal-dependent hydrolase BcerKBAB4_2443 (BcerKBAB4_2443) CSB-YP431513BON
CSB-EP431513BON
CSB-BP431513BON
CSB-MP431513BON
CSB-EP431513BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Kynurenine formamidase (kynB) CSB-YP431514BON
CSB-EP431514BON
CSB-BP431514BON
CSB-MP431514BON
CSB-EP431514BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Putative phosphotransferase BcerKBAB4_4147 (BcerKBAB4_4147) CSB-YP431515BON
CSB-EP431515BON
CSB-BP431515BON
CSB-MP431515BON
CSB-EP431515BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP431516BON
CSB-EP431516BON
CSB-BP431516BON
CSB-MP431516BON
CSB-EP431516BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0297 protein BcerKBAB4_4234 (BcerKBAB4_4234) CSB-YP431517BON
CSB-EP431517BON
CSB-BP431517BON
CSB-MP431517BON
CSB-EP431517BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Lactate utilization protein A 1 (lutA1) CSB-YP431518BON
CSB-EP431518BON
CSB-BP431518BON
CSB-MP431518BON
CSB-EP431518BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Aspartate--tRNA ligase (aspS), partial CSB-YP431519BON
CSB-EP431519BON
CSB-BP431519BON
CSB-MP431519BON
CSB-EP431519BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP431520BON
CSB-EP431520BON
CSB-BP431520BON
CSB-MP431520BON
CSB-EP431520BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0758 protein BcerKBAB4_4299 (BcerKBAB4_4299) CSB-YP431521BON
CSB-EP431521BON
CSB-BP431521BON
CSB-MP431521BON
CSB-EP431521BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Probable GTP-binding protein EngB (engB) CSB-YP431522BON
CSB-EP431522BON
CSB-BP431522BON
CSB-MP431522BON
CSB-EP431522BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP431523BON
CSB-EP431523BON
CSB-BP431523BON
CSB-MP431523BON
CSB-EP431523BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0738 protein BcerKBAB4_1107 (BcerKBAB4_1107) CSB-YP431524BON
CSB-EP431524BON
CSB-BP431524BON
CSB-MP431524BON
CSB-EP431524BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Ribonuclease HIII (rnhC) CSB-YP431525BON
CSB-EP431525BON
CSB-BP431525BON
CSB-MP431525BON
CSB-EP431525BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0477 protein BcerKBAB4_1135 (BcerKBAB4_1135) CSB-YP431526BON
CSB-EP431526BON
CSB-BP431526BON
CSB-MP431526BON
CSB-EP431526BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Lactate utilization protein B 2 (lutB2) CSB-YP431527BON
CSB-EP431527BON
CSB-BP431527BON
CSB-MP431527BON
CSB-EP431527BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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