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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio harveyi Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP417200VEA
CSB-EP417200VEA
CSB-BP417200VEA
CSB-MP417200VEA
CSB-EP417200VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP417201VEA
CSB-EP417201VEA
CSB-BP417201VEA
CSB-MP417201VEA
CSB-EP417201VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP417202VEA
CSB-EP417202VEA
CSB-BP417202VEA
CSB-MP417202VEA
CSB-EP417202VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP417203VEA
CSB-EP417203VEA
CSB-BP417203VEA
CSB-MP417203VEA
CSB-EP417203VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Uridylate kinase (pyrH) CSB-YP417204VEA
CSB-EP417204VEA
CSB-BP417204VEA
CSB-MP417204VEA
CSB-EP417204VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Pyrrolidone-carboxylate peptidase (pcp) CSB-YP417205VEA
CSB-EP417205VEA
CSB-BP417205VEA
CSB-MP417205VEA
CSB-EP417205VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Putative phosphotransferase VIBHAR_06516 (VIBHAR_06516) CSB-YP417206VEA
CSB-EP417206VEA
CSB-BP417206VEA
CSB-MP417206VEA
CSB-EP417206VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Maltoporin (lamB) CSB-YP417207VEA
CSB-EP417207VEA
CSB-BP417207VEA
CSB-MP417207VEA
CSB-EP417207VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi H (+)/Cl (-) exchange transporter ClcA, partial CSB-YP417208VEA
CSB-EP417208VEA
CSB-BP417208VEA
CSB-MP417208VEA
CSB-EP417208VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Periplasmic nitrate reductase (napA), partial CSB-YP417209VEA
CSB-EP417209VEA
CSB-BP417209VEA
CSB-MP417209VEA
CSB-EP417209VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Cobyric acid synthase (cobQ) CSB-YP417210VEA
CSB-EP417210VEA
CSB-BP417210VEA
CSB-MP417210VEA
CSB-EP417210VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP417211FDV
CSB-EP417211FDV
CSB-BP417211FDV
CSB-MP417211FDV
CSB-EP417211FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP417212FDV
CSB-EP417212FDV
CSB-BP417212FDV
CSB-MP417212FDV
CSB-EP417212FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 30S ribosomal protein S2 (rpsB) CSB-YP417213FDV
CSB-EP417213FDV
CSB-BP417213FDV
CSB-MP417213FDV
CSB-EP417213FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 30S ribosomal protein S10 (rpsJ) CSB-YP417214FDV
CSB-EP417214FDV
CSB-BP417214FDV
CSB-MP417214FDV
CSB-EP417214FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 30S ribosomal protein S3 (rpsC) CSB-YP417215FDV
CSB-EP417215FDV
CSB-BP417215FDV
CSB-MP417215FDV
CSB-EP417215FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 50S ribosomal protein L18 (rplR) CSB-YP417216FDV
CSB-EP417216FDV
CSB-BP417216FDV
CSB-MP417216FDV
CSB-EP417216FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 50S ribosomal protein L17 (rplQ) CSB-YP417217FDV
CSB-EP417217FDV
CSB-BP417217FDV
CSB-MP417217FDV
CSB-EP417217FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP417218FDV
CSB-EP417218FDV
CSB-BP417218FDV
CSB-MP417218FDV
CSB-EP417218FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP417219FDV
CSB-EP417219FDV
CSB-BP417219FDV
CSB-MP417219FDV
CSB-EP417219FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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