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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Aspergillus niger ATP-dependent RNA helicase has1 (has1), partial CSB-YP382682AVE
CSB-EP382682AVE
CSB-BP382682AVE
CSB-MP382682AVE
CSB-EP382682AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable beta-galactosidase B (lacB), partial CSB-YP382683AVE
CSB-EP382683AVE
CSB-BP382683AVE
CSB-MP382683AVE
CSB-EP382683AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable 1,4-beta-D-glucan cellobiohydrolase B (cbhB), partial CSB-YP382684AVE
CSB-EP382684AVE
CSB-BP382684AVE
CSB-MP382684AVE
CSB-EP382684AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger PAB-dependent poly (A)-specific ribonuclease subunit pan3, partial CSB-YP382685AVE
CSB-EP382685AVE
CSB-BP382685AVE
CSB-MP382685AVE
CSB-EP382685AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Mitochondrial distribution and morphology protein 12 (mdm12) CSB-YP382686AVE
CSB-EP382686AVE
CSB-BP382686AVE
CSB-MP382686AVE
CSB-EP382686AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger ATP-dependent RNA helicase drs1 (drs1), partial CSB-YP382687AVE
CSB-EP382687AVE
CSB-BP382687AVE
CSB-MP382687AVE
CSB-EP382687AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable endopolygalacturonase E (pgaE) CSB-YP382688AVE
CSB-EP382688AVE
CSB-BP382688AVE
CSB-MP382688AVE
CSB-EP382688AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable endo-1,3 (4)-beta-glucanase An02g00850, partial CSB-YP382689AVE
CSB-EP382689AVE
CSB-BP382689AVE
CSB-MP382689AVE
CSB-EP382689AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Lon protease homolog, mitochondrial (pim1), partial CSB-YP382690AVE
CSB-EP382690AVE
CSB-BP382690AVE
CSB-MP382690AVE
CSB-EP382690AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable endopolygalacturonase B (pgaB) CSB-YP382691AVE
CSB-EP382691AVE
CSB-BP382691AVE
CSB-MP382691AVE
CSB-EP382691AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Mannitol-1-phosphate 5-dehydrogenase (mpdA) CSB-YP382692AVE
CSB-EP382692AVE
CSB-BP382692AVE
CSB-MP382692AVE
CSB-EP382692AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger ATP-dependent rRNA helicase spb4 (spb4), partial CSB-YP382693AVE
CSB-EP382693AVE
CSB-BP382693AVE
CSB-MP382693AVE
CSB-EP382693AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger ATP-dependent RNA helicase eIF4A (tif1) CSB-YP382694AVE
CSB-EP382694AVE
CSB-BP382694AVE
CSB-MP382694AVE
CSB-EP382694AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable exopolygalacturonase C (pgxC) CSB-YP382695AVE
CSB-EP382695AVE
CSB-BP382695AVE
CSB-MP382695AVE
CSB-EP382695AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger ATP-dependent RNA helicase dbp3 (dbp3) CSB-YP382696AVE
CSB-EP382696AVE
CSB-BP382696AVE
CSB-MP382696AVE
CSB-EP382696AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable endo-1,4-beta-xylanase C (xlnC) CSB-YP382697AVE
CSB-EP382697AVE
CSB-BP382697AVE
CSB-MP382697AVE
CSB-EP382697AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Alpha-L-rhamnosidase rgxB (rgxB) CSB-YP382698AVE
CSB-EP382698AVE
CSB-BP382698AVE
CSB-MP382698AVE
CSB-EP382698AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable Xaa-Pro aminopeptidase P (ampp), partial CSB-YP382699AVE
CSB-EP382699AVE
CSB-BP382699AVE
CSB-MP382699AVE
CSB-EP382699AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable glucan endo-1,3-beta-glucosidase eglC (eglC) CSB-YP382700AVE
CSB-EP382700AVE
CSB-BP382700AVE
CSB-MP382700AVE
CSB-EP382700AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Protein get1 (get1), partial CSB-YP382701AVE1
CSB-EP382701AVE1
CSB-BP382701AVE1
CSB-MP382701AVE1
CSB-EP382701AVE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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