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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella amazonensis 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP376415STK
CSB-EP376415STK
CSB-BP376415STK
CSB-MP376415STK
CSB-EP376415STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Integration host factor subunit alpha (ihfA) CSB-YP376416STK
CSB-EP376416STK
CSB-BP376416STK
CSB-MP376416STK
CSB-EP376416STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Biosynthetic arginine decarboxylase (speA), partial CSB-YP376417STK
CSB-EP376417STK
CSB-BP376417STK
CSB-MP376417STK
CSB-EP376417STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Phosphate acyltransferase (plsX) CSB-YP376418STK
CSB-EP376418STK
CSB-BP376418STK
CSB-MP376418STK
CSB-EP376418STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Deoxycytidine triphosphate deaminase (dcd) CSB-YP376419STK
CSB-EP376419STK
CSB-BP376419STK
CSB-MP376419STK
CSB-EP376419STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Translation initiation factor IF-1 (infA) CSB-YP376420STK
CSB-EP376420STK
CSB-BP376420STK
CSB-MP376420STK
CSB-EP376420STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP376421STK
CSB-EP376421STK
CSB-BP376421STK
CSB-MP376421STK
CSB-EP376421STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Uracil phosphoribosyltransferase (upp) CSB-YP376422STK
CSB-EP376422STK
CSB-BP376422STK
CSB-MP376422STK
CSB-EP376422STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Tryptophan synthase beta chain (trpB) CSB-YP376423STK
CSB-EP376423STK
CSB-BP376423STK
CSB-MP376423STK
CSB-EP376423STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP376424STK
CSB-EP376424STK
CSB-BP376424STK
CSB-MP376424STK
CSB-EP376424STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP376425STK
CSB-EP376425STK
CSB-BP376425STK
CSB-MP376425STK
CSB-EP376425STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Glutamate--tRNA ligase (gltX) CSB-YP376426STK
CSB-EP376426STK
CSB-BP376426STK
CSB-MP376426STK
CSB-EP376426STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Putative pterin-4-alpha-carbinolamine dehydratase (Sama_2221) CSB-YP376427STK
CSB-EP376427STK
CSB-BP376427STK
CSB-MP376427STK
CSB-EP376427STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Flagellar P-ring protein (flgI) CSB-YP376428STK
CSB-EP376428STK
CSB-BP376428STK
CSB-MP376428STK
CSB-EP376428STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Histidine--tRNA ligase (hisS) CSB-YP376429STK
CSB-EP376429STK
CSB-BP376429STK
CSB-MP376429STK
CSB-EP376429STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP376430STK
CSB-EP376430STK
CSB-BP376430STK
CSB-MP376430STK
CSB-EP376430STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP376431STK
CSB-EP376431STK
CSB-BP376431STK
CSB-MP376431STK
CSB-EP376431STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Protein grpE (grpE) CSB-YP376432STK
CSB-EP376432STK
CSB-BP376432STK
CSB-MP376432STK
CSB-EP376432STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis UPF0235 protein Sama_2480 (Sama_2480) CSB-YP376433STK
CSB-EP376433STK
CSB-BP376433STK
CSB-MP376433STK
CSB-EP376433STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Probable Fe (2+)-trafficking protein CSB-YP376434STK
CSB-EP376434STK
CSB-BP376434STK
CSB-MP376434STK
CSB-EP376434STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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