重組蛋白
重組蛋白是解析生物相互作用的關(guān)鍵工具，廣泛應(yīng)用于各類研究實驗中。無論是商業(yè)化產(chǎn)品，還是為特定研究定制的蛋白，它們都在多種科學應(yīng)用中發(fā)揮著不可替代的作用。
若您需要優(yōu)質(zhì)重組蛋白——已驗證的生物活性、低內(nèi)毒素水平、高純度——來為您的研究提供可靠支持，這里正是您的理想之選。
歷經(jīng)18年積累，華美生物已構(gòu)建覆蓋原核與真核的五大蛋白質(zhì)表達系統(tǒng)。我們擁有包括桿狀病毒外膜蛋白（OMP）表達技術(shù)、膜蛋白表達技術(shù)等在內(nèi)的多項蛋白表達與純化專有技術(shù)，致力于為您提供高質(zhì)量的重組蛋白解決方案。
產(chǎn)品特點
- 覆蓋廣泛：擁有超過16,000種重組蛋白，涵蓋人類、小鼠、大鼠、兔、豚鼠、雞、猴、豬等多種物種的基因與靶點，支持跨物種比較研究。
- 表達系統(tǒng)全面：提供五大表達系統(tǒng)，包括大腸桿菌、酵母、桿狀病毒-昆蟲細胞、哺乳動物細胞以及特有的無細胞表達系統(tǒng)。該系統(tǒng)省去質(zhì)粒轉(zhuǎn)化、細胞培養(yǎng)、破碎離心等步驟，顯著提升蛋白制備效率。
- 多標簽選擇：支持His、GST、Flag、MBP等多種標簽，便于后續(xù)純化、檢測與功能研究。
- 多領(lǐng)域應(yīng)用：適用于抗原制備、細胞與分析、蛋白互作、藥物研發(fā)、酶活性檢測、ELISA標準品、體內(nèi)外研究、質(zhì)譜與結(jié)構(gòu)分析等廣泛場景。
- 無動物源成分：我們已建立全程無動物副產(chǎn)品接觸的重組蛋白生產(chǎn)線，確保產(chǎn)品的高純凈性與一致性，適用于對來源敏感的各類實驗。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì)，或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的重組蛋白，請查看華美生物蛋白定制服務(wù)。
通過選擇目標名稱的首字母瀏覽產(chǎn)品。
| 產(chǎn)品名稱 | 貨號 | 規(guī)格 |
|---|---|---|
| Recombinant Paracoccus denitrificans Leucyl/phenylalanyl-tRNA--protein transferase (aat) | CSB-YP373534PYC CSB-EP373534PYC CSB-BP373534PYC CSB-MP373534PYC CSB-EP373534PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans Proline--tRNA ligase (proS) | CSB-YP373535PYC CSB-EP373535PYC CSB-BP373535PYC CSB-MP373535PYC CSB-EP373535PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans Carboxylate-amine ligase Pden_1633 (Pden_1633) | CSB-YP373536PYC CSB-EP373536PYC CSB-BP373536PYC CSB-MP373536PYC CSB-EP373536PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) | CSB-YP373537PYC CSB-EP373537PYC CSB-BP373537PYC CSB-MP373537PYC CSB-EP373537PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans Ribulose bisphosphate carboxylase large chain (cbbL) | CSB-YP373538PYC CSB-EP373538PYC CSB-BP373538PYC CSB-MP373538PYC CSB-EP373538PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) | CSB-YP373539PYC CSB-EP373539PYC CSB-BP373539PYC CSB-MP373539PYC CSB-EP373539PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans Phosphate acyltransferase (plsX) | CSB-YP373540PYC CSB-EP373540PYC CSB-BP373540PYC CSB-MP373540PYC CSB-EP373540PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans S-adenosylmethionine synthase (metK) | CSB-YP373541PYC CSB-EP373541PYC CSB-BP373541PYC CSB-MP373541PYC CSB-EP373541PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans Imidazole glycerol phosphate synthase subunit HisF (hisF) | CSB-YP373542PYC CSB-EP373542PYC CSB-BP373542PYC CSB-MP373542PYC CSB-EP373542PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans UPF0314 protein Pden_1914 (Pden_1914), partial | CSB-YP373543PYC1 CSB-EP373543PYC1 CSB-BP373543PYC1 CSB-MP373543PYC1 CSB-EP373543PYC1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) | CSB-YP373544PYC CSB-EP373544PYC CSB-BP373544PYC CSB-MP373544PYC CSB-EP373544PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans Ribosomal protein S12 methylthiotransferase RimO (rimO) | CSB-YP373545PYC CSB-EP373545PYC CSB-BP373545PYC CSB-MP373545PYC CSB-EP373545PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans Tetraacyldisaccharide 4'-kinase (lpxK) | CSB-YP373546PYC CSB-EP373546PYC CSB-BP373546PYC CSB-MP373546PYC CSB-EP373546PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans Anthranilate phosphoribosyltransferase (trpD) | CSB-YP373547PYC CSB-EP373547PYC CSB-BP373547PYC CSB-MP373547PYC CSB-EP373547PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans Aspartate--tRNA ligase (aspS), partial | CSB-YP373548PYC CSB-EP373548PYC CSB-BP373548PYC CSB-MP373548PYC CSB-EP373548PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans Type III pantothenate kinase (coaX) | CSB-YP373549PYC CSB-EP373549PYC CSB-BP373549PYC CSB-MP373549PYC CSB-EP373549PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans NADH-quinone oxidoreductase subunit H (nuoH), partial | CSB-YP373550PYC CSB-EP373550PYC CSB-BP373550PYC CSB-MP373550PYC CSB-EP373550PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans Large-conductance mechanosensitive channel (mscL), partial | CSB-YP373551PYC1 CSB-EP373551PYC1 CSB-BP373551PYC1 CSB-MP373551PYC1 CSB-EP373551PYC1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans 50S ribosomal protein L35 (rpmI) | CSB-YP373552PYC CSB-EP373552PYC CSB-BP373552PYC CSB-MP373552PYC CSB-EP373552PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Paracoccus denitrificans Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) | CSB-YP373553PYC CSB-EP373553PYC CSB-BP373553PYC CSB-MP373553PYC CSB-EP373553PYC-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。
第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條：如何選擇合適的表達載體?
表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。
根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022
