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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human Neurogenic locus notch homolog protein 1 (NOTCH1), partial CSB-MP015949HU1 1mg/100ug/20ug
Recombinant Human Cellular tumor antigen p53 (TP53) CSB-MP024077HU 1mg/100ug/20ug
Recombinant Human Killer cell immunoglobulin-like receptor 2DL3 (KIR2DL3), partial CSB-MP012354HU 1mg/100ug/20ug
Recombinant Human HERV-H_2q24.3 provirus ancestral Env polyprotein, partial CSB-MP885640HU 1mg/100ug/20ug
Recombinant Human High affinity immunoglobulin epsilon receptor subunit alpha (FCER1A), partial CSB-MP008532HU 1mg/100ug/20ug
Recombinant Human Hepatitis A virus cellular receptor 1 (HAVCR1), partial CSB-MP010144HU1 1mg/100ug/20ug
Recombinant Human Interleukin-12 subunit beta (IL12B) CSB-MP011587HUd7 1mg/100ug/20ug
Recombinant Human Protein NDRG1 (NDRG1)(S336D,T346D) CSB-MP835678HU(M) 1mg/100ug/20ug
Recombinant Human TP53&S100P Heterodimer Protein CSB-MP7721HU 1mg/100ug/20ug
Recombinant Human C-X-C motif chemokine 5 (CXCL5) CSB-MP006250HU1(A4) 1mg/100ug/20ug
Recombinant Mouse Tumor necrosis factor ligand superfamily member 13B (Tnfsf13b), partial CSB-MP894359MO 1mg/100ug/20ug
Recombinant Mouse Tumor necrosis factor receptor superfamily member 13C (Tnfrsf13c), partial CSB-MP884026MO 1mg/100ug/20ug
Recombinant Human Receptor-type tyrosine-protein kinase FLT3 (FLT3), partial, Biotinylated CSB-MP008733HU-B 1mg/100ug/20ug
Recombinant Human Galectin-3 (LGALS3) CSB-MP012887HU(A4)d7 1mg/100ug/20ug
Recombinant Human IL27B&IL27A Heterodimer Protein CSB-MP5979HU 1mg/100ug/20ug
Recombinant Human Endoplasmic reticulum resident protein 29 (ERP29) CSB-YP007800HU 1mg/100ug/20ug
Recombinant Candida albicans Cell surface mannoprotein MP65 (MP65), partial CSB-YP3653CZD 1mg/100ug/20ug
Recombinant Human Endoplasmic reticulum resident protein 29 (ERP29) CSB-YP007800HUa4 1mg/100ug/20ug
Recombinant Lassa virus Pre-glycoprotein polyprotein GP complex (GPC), partial CSB-YP362480LNP2 1mg/100ug/20ug
Recombinant Horse Myelin P2 protein (PMP2) CSB-YP315130HO 1mg/100ug/20ug

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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