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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Putative membrane metalloprotease yxkI (yxkI), partial CSB-YP310940BRJ
CSB-EP310940BRJ
CSB-BP310940BRJ
CSB-MP310940BRJ
CSB-EP310940BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis ATP-binding/permease protein CydC (cydC), partial CSB-YP310941BRJ1
CSB-EP310941BRJ1
CSB-BP310941BRJ1
CSB-MP310941BRJ1
CSB-EP310941BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter yxlH (yxlH), partial CSB-YP310942BRJ
CSB-EP310942BRJ
CSB-BP310942BRJ
CSB-MP310942BRJ
CSB-EP310942BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Cephalosporin C deacetylase (cah) CSB-YP310943BRJ
CSB-EP310943BRJ
CSB-BP310943BRJ
CSB-MP310943BRJ
CSB-EP310943BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0703 protein ycgQ (ycgQ), partial CSB-YP310944BRJ1
CSB-EP310944BRJ1
CSB-BP310944BRJ1
CSB-MP310944BRJ1
CSB-EP310944BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized transporter yclF (yclF), partial CSB-YP310945BRJ
CSB-EP310945BRJ
CSB-BP310945BRJ
CSB-MP310945BRJ
CSB-EP310945BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter permease protein yclO (yclO), partial CSB-YP310946BRJ
CSB-EP310946BRJ
CSB-BP310946BRJ
CSB-MP310946BRJ
CSB-EP310946BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional repressor ycnK (ycnK) CSB-YP310947BRJ
CSB-EP310947BRJ
CSB-BP310947BRJ
CSB-MP310947BRJ
CSB-EP310947BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized transcriptional regulatory protein yfiK (yfiK) CSB-YP310948BRJ
CSB-EP310948BRJ
CSB-BP310948BRJ
CSB-MP310948BRJ
CSB-EP310948BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable transcriptional regulatory protein YrbC (yrbC) CSB-YP310949BRJ
CSB-EP310949BRJ
CSB-BP310949BRJ
CSB-MP310949BRJ
CSB-EP310949BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Levanbiose-producing levanase (levB) CSB-YP310950GFM
CSB-EP310950GFM
CSB-BP310950GFM
CSB-MP310950GFM
CSB-EP310950GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yebA (yebA), partial CSB-YP310951BRJ
CSB-EP310951BRJ
CSB-BP310951BRJ
CSB-MP310951BRJ
CSB-EP310951BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Beta-xylosidase (xynB), partial CSB-YP310952BRJ
CSB-EP310952BRJ
CSB-BP310952BRJ
CSB-MP310952BRJ
CSB-EP310952BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Alanine racemase 2 (alr2) CSB-YP310953BRJ
CSB-EP310953BRJ
CSB-BP310953BRJ
CSB-MP310953BRJ
CSB-EP310953BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable tautomerase yrdN (yrdN) CSB-YP310954BRJ
CSB-EP310954BRJ
CSB-BP310954BRJ
CSB-MP310954BRJ
CSB-EP310954BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sensor protein lytS (lytS), partial CSB-YP310955BRJ1
CSB-EP310955BRJ1
CSB-BP310955BRJ1
CSB-MP310955BRJ1
CSB-EP310955BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yscB (yscB) CSB-YP310956BRJ
CSB-EP310956BRJ
CSB-BP310956BRJ
CSB-MP310956BRJ
CSB-EP310956BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glycerol-1-phosphate dehydrogenase [NAD (P)+] CSB-YP310957BRJ
CSB-EP310957BRJ
CSB-BP310957BRJ
CSB-MP310957BRJ
CSB-EP310957BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glycolate oxidase subunit glcD (glcD) CSB-YP310958BRJ
CSB-EP310958BRJ
CSB-BP310958BRJ
CSB-MP310958BRJ
CSB-EP310958BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Cell division protein ZapA (zapA) CSB-YP310959BRJ
CSB-EP310959BRJ
CSB-BP310959BRJ
CSB-MP310959BRJ
CSB-EP310959BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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