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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Listeria monocytogenes serovar 1/2a 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP841466LPY
CSB-EP841466LPY
CSB-BP841466LPY
CSB-MP841466LPY
CSB-EP841466LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP841467LPY
CSB-EP841467LPY
CSB-BP841467LPY
CSB-MP841467LPY
CSB-EP841467LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Foldase protein prsA 2 (prsA2) CSB-YP841468LPY
CSB-EP841468LPY
CSB-BP841468LPY
CSB-MP841468LPY
CSB-EP841468LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Phosphoglucosamine mutase (glmM) CSB-YP841469LPY
CSB-EP841469LPY
CSB-BP841469LPY
CSB-MP841469LPY
CSB-EP841469LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP841470LPY
CSB-EP841470LPY
CSB-BP841470LPY
CSB-MP841470LPY
CSB-EP841470LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Protein MraZ (mraZ) CSB-YP841471LPY
CSB-EP841471LPY
CSB-BP841471LPY
CSB-MP841471LPY
CSB-EP841471LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Quinolinate synthase A (nadA) CSB-YP841472LPY
CSB-EP841472LPY
CSB-BP841472LPY
CSB-MP841472LPY
CSB-EP841472LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a 3-isopropylmalate dehydrogenase (leuB) CSB-YP841473LPY
CSB-EP841473LPY
CSB-BP841473LPY
CSB-MP841473LPY
CSB-EP841473LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Ferredoxin--NADP reductase 1 (lmo1961) CSB-YP841474LPY
CSB-EP841474LPY
CSB-BP841474LPY
CSB-MP841474LPY
CSB-EP841474LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Nucleoside diphosphate kinase (ndk) CSB-YP841475LPY
CSB-EP841475LPY
CSB-BP841475LPY
CSB-MP841475LPY
CSB-EP841475LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Dihydrodipicolinate reductase (dapB) CSB-YP841476LPY
CSB-EP841476LPY
CSB-BP841476LPY
CSB-MP841476LPY
CSB-EP841476LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a UPF0398 protein lmo1889 (lmo1889) CSB-YP841477LPY
CSB-EP841477LPY
CSB-BP841477LPY
CSB-MP841477LPY
CSB-EP841477LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP841478LPY
CSB-EP841478LPY
CSB-BP841478LPY
CSB-MP841478LPY
CSB-EP841478LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Lipoprotein signal peptidase (lspA), partial CSB-YP841479LPY1
CSB-EP841479LPY1
CSB-BP841479LPY1
CSB-MP841479LPY1
CSB-EP841479LPY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Guanylate kinase (gmk) CSB-YP841480LPY
CSB-EP841480LPY
CSB-BP841480LPY
CSB-MP841480LPY
CSB-EP841480LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a UPF0122 protein lmo1802 (lmo1802) CSB-YP841481LPY
CSB-EP841481LPY
CSB-BP841481LPY
CSB-MP841481LPY
CSB-EP841481LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP841482LPY
CSB-EP841482LPY
CSB-BP841482LPY
CSB-MP841482LPY
CSB-EP841482LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Adenine deaminase (ade), partial CSB-YP841483LPY
CSB-EP841483LPY
CSB-BP841483LPY
CSB-MP841483LPY
CSB-EP841483LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP841484LPY
CSB-EP841484LPY
CSB-BP841484LPY
CSB-MP841484LPY
CSB-EP841484LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Tryptophan synthase beta chain (trpB) CSB-YP841485LPY
CSB-EP841485LPY
CSB-BP841485LPY
CSB-MP841485LPY
CSB-EP841485LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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