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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Coxiella burnetii Shikimate dehydrogenase (aroE) CSB-YP769391DXP
CSB-EP769391DXP
CSB-BP769391DXP
CSB-MP769391DXP
CSB-EP769391DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Probable histidine--tRNA ligase (hisS) CSB-YP769392TIW
CSB-EP769392TIW
CSB-BP769392TIW
CSB-MP769392TIW
CSB-EP769392TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Glutamyl-tRNA reductase (hemA) CSB-YP769393TIW
CSB-EP769393TIW
CSB-BP769393TIW
CSB-MP769393TIW
CSB-EP769393TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei UPF0336 protein TWT_721 (TWT_721) CSB-YP769394TIW
CSB-EP769394TIW
CSB-BP769394TIW
CSB-MP769394TIW
CSB-EP769394TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 30S ribosomal protein S7 (rpsG) CSB-YP769395TIW
CSB-EP769395TIW
CSB-BP769395TIW
CSB-MP769395TIW
CSB-EP769395TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP769396TIW
CSB-EP769396TIW
CSB-BP769396TIW
CSB-MP769396TIW
CSB-EP769396TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP769397TIW
CSB-EP769397TIW
CSB-BP769397TIW
CSB-MP769397TIW
CSB-EP769397TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 50S ribosomal protein L2 (rplB) CSB-YP769398TIW
CSB-EP769398TIW
CSB-BP769398TIW
CSB-MP769398TIW
CSB-EP769398TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 50S ribosomal protein L24 (rplX) CSB-YP769399TIW
CSB-EP769399TIW
CSB-BP769399TIW
CSB-MP769399TIW
CSB-EP769399TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 50S ribosomal protein L30 (rpmD) CSB-YP769400TIW
CSB-EP769400TIW
CSB-BP769400TIW
CSB-MP769400TIW
CSB-EP769400TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei tRNA pseudouridine synthase A (truA) CSB-YP769401TIW
CSB-EP769401TIW
CSB-BP769401TIW
CSB-MP769401TIW
CSB-EP769401TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP769402TIW
CSB-EP769402TIW
CSB-BP769402TIW
CSB-MP769402TIW
CSB-EP769402TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP769403TIW
CSB-EP769403TIW
CSB-BP769403TIW
CSB-MP769403TIW
CSB-EP769403TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei ATP synthase subunit b (atpF), partial CSB-YP769404TIW1
CSB-EP769404TIW1
CSB-BP769404TIW1
CSB-MP769404TIW1
CSB-EP769404TIW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA), partial CSB-YP769405TIW
CSB-EP769405TIW
CSB-BP769405TIW
CSB-MP769405TIW
CSB-EP769405TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Leucine--tRNA ligase (leuS), partial CSB-YP769406TIW
CSB-EP769406TIW
CSB-BP769406TIW
CSB-MP769406TIW
CSB-EP769406TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Guanylate kinase (gmk) CSB-YP769407TIW
CSB-EP769407TIW
CSB-BP769407TIW
CSB-MP769407TIW
CSB-EP769407TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Peptide deformylase (def) CSB-YP769408TIW
CSB-EP769408TIW
CSB-BP769408TIW
CSB-MP769408TIW
CSB-EP769408TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 30S ribosomal protein S20 (rpsT) CSB-YP769409TIW
CSB-EP769409TIW
CSB-BP769409TIW
CSB-MP769409TIW
CSB-EP769409TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP769410TIW
CSB-EP769410TIW
CSB-BP769410TIW
CSB-MP769410TIW
CSB-EP769410TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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