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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. japonica Homeobox-leucine zipper protein HOX24 (HOX24) CSB-YP760703OFG
CSB-EP760703OFG
CSB-BP760703OFG
CSB-MP760703OFG
CSB-EP760703OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Chaperone protein ClpD1, chloroplastic (CLPD1), partial CSB-YP760704OFG
CSB-EP760704OFG
CSB-BP760704OFG
CSB-MP760704OFG
CSB-EP760704OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Zinc finger CCCH domain-containing protein 13 (Os02g0161200, LOC_Os02g06584) CSB-YP760705OFG
CSB-EP760705OFG
CSB-BP760705OFG
CSB-MP760705OFG
CSB-EP760705OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II D2 protein (psbD), partial CSB-YP760706SHAC1
CSB-EP760706SHAC1
CSB-BP760706SHAC1
CSB-MP760706SHAC1
CSB-EP760706SHAC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equisetum variegatum 30S ribosomal protein S4, chloroplastic (rps4) CSB-YP760707ECAZ
CSB-EP760707ECAZ
CSB-BP760707ECAZ
CSB-MP760707ECAZ
CSB-EP760707ECAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Astacin-like metalloendopeptidase (ASTL) CSB-YP760708HU
CSB-MP760708HU
CSB-EP760708HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Thioredoxin reductase, mitochondrial (TRR1) CSB-YP760709KBK
CSB-EP760709KBK
CSB-BP760709KBK
CSB-MP760709KBK
CSB-EP760709KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP760710BAAE
CSB-EP760710BAAE
CSB-BP760710BAAE
CSB-MP760710BAAE
CSB-EP760710BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian GMP reductase (guaC) CSB-YP760711BAAE
CSB-EP760711BAAE
CSB-BP760711BAAE
CSB-MP760711BAAE
CSB-EP760711BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Serine hydroxymethyltransferase (glyA) CSB-YP760712BAAE
CSB-EP760712BAAE
CSB-BP760712BAAE
CSB-MP760712BAAE
CSB-EP760712BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian ATP synthase subunit alpha (atpA), partial CSB-YP760713BAAE
CSB-EP760713BAAE
CSB-BP760713BAAE
CSB-MP760713BAAE
CSB-EP760713BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP760714BAAE1
CSB-EP760714BAAE1
CSB-BP760714BAAE1
CSB-MP760714BAAE1
CSB-EP760714BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UvrABC system protein B (uvrB), partial CSB-YP760715BAAE
CSB-EP760715BAAE
CSB-BP760715BAAE
CSB-MP760715BAAE
CSB-EP760715BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0042 nucleotide-binding protein BT9727_4833 (BT9727_4833) CSB-YP760716BAAE
CSB-EP760716BAAE
CSB-BP760716BAAE
CSB-MP760716BAAE
CSB-EP760716BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian SsrA-binding protein (smpB) CSB-YP760717BAAE
CSB-EP760717BAAE
CSB-BP760717BAAE
CSB-MP760717BAAE
CSB-EP760717BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Tyrosine--tRNA ligase 2 (tyrS2) CSB-YP760718BAAE
CSB-EP760718BAAE
CSB-BP760718BAAE
CSB-MP760718BAAE
CSB-EP760718BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian L-lactate dehydrogenase 2 (ldh2) CSB-YP760719BAAE
CSB-EP760719BAAE
CSB-BP760719BAAE
CSB-MP760719BAAE
CSB-EP760719BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian S-adenosylmethionine synthase (metK) CSB-YP760720BAAE
CSB-EP760720BAAE
CSB-BP760720BAAE
CSB-MP760720BAAE
CSB-EP760720BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S4 (rpsD) CSB-YP760721BAAE
CSB-EP760721BAAE
CSB-BP760721BAAE
CSB-MP760721BAAE
CSB-EP760721BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0173 metal-dependent hydrolase BT9727_4343 (BT9727_4343) CSB-YP760722BAAE
CSB-EP760722BAAE
CSB-BP760722BAAE
CSB-MP760722BAAE
CSB-EP760722BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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