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Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian N- (5'-phosphoribosyl)anthranilate isomerase (trpF) CSB-YP750295BAAE
CSB-EP750295BAAE
CSB-BP750295BAAE
CSB-MP750295BAAE
CSB-EP750295BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0738 protein BT9727_1100 (BT9727_1100) CSB-YP750296BAAE
CSB-EP750296BAAE
CSB-BP750296BAAE
CSB-MP750296BAAE
CSB-EP750296BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ferrochelatase 2 (hemH2) CSB-YP750297BAAE
CSB-EP750297BAAE
CSB-BP750297BAAE
CSB-MP750297BAAE
CSB-EP750297BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ferrochelatase 1 (hemH1) CSB-YP750298BAAE
CSB-EP750298BAAE
CSB-BP750298BAAE
CSB-MP750298BAAE
CSB-EP750298BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0754 membrane protein BT9727_0767 (BT9727_0767), partial CSB-YP750299BAAE1
CSB-EP750299BAAE1
CSB-BP750299BAAE1
CSB-MP750299BAAE1
CSB-EP750299BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian D-ribose pyranase (rbsD) CSB-YP750300BAAE
CSB-EP750300BAAE
CSB-BP750300BAAE
CSB-MP750300BAAE
CSB-EP750300BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0435 protein BT9727_0383 (BT9727_0383) CSB-YP750301BAAE
CSB-EP750301BAAE
CSB-BP750301BAAE
CSB-MP750301BAAE
CSB-EP750301BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Arginine regulator (argR1) CSB-YP750302BAAE
CSB-EP750302BAAE
CSB-BP750302BAAE
CSB-MP750302BAAE
CSB-EP750302BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP750303BAAE
CSB-EP750303BAAE
CSB-BP750303BAAE
CSB-MP750303BAAE
CSB-EP750303BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L13 (rplM) CSB-YP750304BAAE
CSB-EP750304BAAE
CSB-BP750304BAAE
CSB-MP750304BAAE
CSB-EP750304BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S13 (rpsM) CSB-YP750305BAAE
CSB-EP750305BAAE
CSB-BP750305BAAE
CSB-MP750305BAAE
CSB-EP750305BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S17 (rpsQ) CSB-YP750306BAAE
CSB-EP750306BAAE
CSB-BP750306BAAE
CSB-MP750306BAAE
CSB-EP750306BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L1 (rplA) CSB-YP750307BAAE
CSB-EP750307BAAE
CSB-BP750307BAAE
CSB-MP750307BAAE
CSB-EP750307BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Lysine--tRNA ligase (lysS) CSB-YP750308BAAE
CSB-EP750308BAAE
CSB-BP750308BAAE
CSB-MP750308BAAE
CSB-EP750308BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0213 protein BT9727_0031 (BT9727_0031) CSB-YP750309BAAE
CSB-EP750309BAAE
CSB-BP750309BAAE
CSB-MP750309BAAE
CSB-EP750309BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP750310BAAE
CSB-EP750310BAAE
CSB-BP750310BAAE
CSB-MP750310BAAE
CSB-EP750310BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA pseudouridine synthase A 2 (truA2) CSB-YP750311BQE
CSB-EP750311BQE
CSB-BP750311BQE
CSB-MP750311BQE
CSB-EP750311BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cysteine proteinase inhibitor 3 (Os05g0409300, LOC_Os05g33880) CSB-YP750312OFG
CSB-EP750312OFG
CSB-BP750312OFG
CSB-MP750312OFG
CSB-EP750312OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica BURP domain-containing protein 2 (BURP2) CSB-YP750313OFG
CSB-EP750313OFG
CSB-BP750313OFG
CSB-MP750313OFG
CSB-EP750313OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Glutamine-dependent NAD (+) synthetase (NADSYN1) CSB-YP750314HU
CSB-EP750314HU
CSB-BP750314HU
CSB-MP750314HU
CSB-EP750314HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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