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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ashbya gossypii Crossover junction endonuclease MUS81 (MUS81), partial CSB-YP741604DOT
CSB-EP741604DOT
CSB-BP741604DOT
CSB-MP741604DOT
CSB-EP741604DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Increased rDNA silencing protein 4 (IRS4), partial CSB-YP741605DOT
CSB-EP741605DOT
CSB-BP741605DOT
CSB-MP741605DOT
CSB-EP741605DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pheromone-regulated membrane protein 10 (AFR075C), partial CSB-YP741606DOT
CSB-EP741606DOT
CSB-BP741606DOT
CSB-MP741606DOT
CSB-EP741606DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Acetyl-CoA hydrolase (ACH1), partial CSB-YP741607DOT
CSB-EP741607DOT
CSB-BP741607DOT
CSB-MP741607DOT
CSB-EP741607DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Chromatin structure-remodeling complex subunit SFH1 (SFH1) CSB-YP741608DOT
CSB-EP741608DOT
CSB-BP741608DOT
CSB-MP741608DOT
CSB-EP741608DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Transcription activator of gluconeogenesis ERT1 (ERT1), partial CSB-YP741609DOT
CSB-EP741609DOT
CSB-BP741609DOT
CSB-MP741609DOT
CSB-EP741609DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Biogenesis of lysosome-related organelles complex 1 subunit KXD1 (KXD1) CSB-YP741610DOT
CSB-EP741610DOT
CSB-BP741610DOT
CSB-MP741610DOT
CSB-EP741610DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP-dependent RNA helicase MRH4, mitochondrial (MRH4) CSB-YP741611DOT
CSB-EP741611DOT
CSB-BP741611DOT
CSB-MP741611DOT
CSB-EP741611DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii V-type proton ATPase 16 kDa proteolipid subunit 2 (VMA11), partial CSB-YP741612DOT1
CSB-EP741612DOT1
CSB-BP741612DOT1
CSB-MP741612DOT1
CSB-EP741612DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Vacuolar protein sorting-associated protein 27 (VPS27), partial CSB-YP741613DOT
CSB-EP741613DOT
CSB-BP741613DOT
CSB-MP741613DOT
CSB-EP741613DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP-dependent RNA helicase DBP2 (DBP2), partial CSB-YP741614DOT
CSB-EP741614DOT
CSB-BP741614DOT
CSB-MP741614DOT
CSB-EP741614DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial distribution and morphology protein 34 (MDM34), partial CSB-YP741615DOT
CSB-EP741615DOT
CSB-BP741615DOT
CSB-MP741615DOT
CSB-EP741615DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Autophagy-related protein 8 (ATG8) CSB-YP741616DOT
CSB-EP741616DOT
CSB-BP741616DOT
CSB-MP741616DOT
CSB-EP741616DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Cap-associated protein CAF20 (CAF20) CSB-YP741617DOT
CSB-EP741617DOT
CSB-BP741617DOT
CSB-MP741617DOT
CSB-EP741617DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI19 (GPI19), partial CSB-YP741618DOT1
CSB-EP741618DOT1
CSB-BP741618DOT1
CSB-MP741618DOT1
CSB-EP741618DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP-dependent RNA helicase DBP8 (DBP8) CSB-YP741619DOT
CSB-EP741619DOT
CSB-BP741619DOT
CSB-MP741619DOT
CSB-EP741619DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein ESC2 (ESC2) CSB-YP741620DOT
CSB-EP741620DOT
CSB-BP741620DOT
CSB-MP741620DOT
CSB-EP741620DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Phosphatidylglycerol/phosphatidylinositol transfer protein (NPC2) CSB-YP741621DOT
CSB-EP741621DOT
CSB-BP741621DOT
CSB-MP741621DOT
CSB-EP741621DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii FACT complex subunit POB3 (POB3), partial CSB-YP741622DOT
CSB-EP741622DOT
CSB-BP741622DOT
CSB-MP741622DOT
CSB-EP741622DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Synaptobrevin homolog YKT6 (YKT6) CSB-YP741623DOT
CSB-EP741623DOT
CSB-BP741623DOT
CSB-MP741623DOT
CSB-EP741623DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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