重組蛋白
重組蛋白是解析生物相互作用的關(guān)鍵工具，廣泛應(yīng)用于各類研究實(shí)驗(yàn)中。無(wú)論是商業(yè)化產(chǎn)品，還是為特定研究定制的蛋白，它們都在多種科學(xué)應(yīng)用中發(fā)揮著不可替代的作用。
若您需要優(yōu)質(zhì)重組蛋白——已驗(yàn)證的生物活性、低內(nèi)毒素水平、高純度——來(lái)為您的研究提供可靠支持，這里正是您的理想之選。
歷經(jīng)18年積累，華美生物已構(gòu)建覆蓋原核與真核的五大蛋白質(zhì)表達(dá)系統(tǒng)。我們擁有包括桿狀病毒外膜蛋白（OMP）表達(dá)技術(shù)、膜蛋白表達(dá)技術(shù)等在內(nèi)的多項(xiàng)蛋白表達(dá)與純化專有技術(shù)，致力于為您提供高質(zhì)量的重組蛋白解決方案。
產(chǎn)品特點(diǎn)
- 覆蓋廣泛：擁有超過(guò)16,000種重組蛋白，涵蓋人類、小鼠、大鼠、兔、豚鼠、雞、猴、豬等多種物種的基因與靶點(diǎn)，支持跨物種比較研究。
- 表達(dá)系統(tǒng)全面：提供五大表達(dá)系統(tǒng)，包括大腸桿菌、酵母、桿狀病毒-昆蟲細(xì)胞、哺乳動(dòng)物細(xì)胞以及特有的無(wú)細(xì)胞表達(dá)系統(tǒng)。該系統(tǒng)省去質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、破碎離心等步驟，顯著提升蛋白制備效率。
- 多標(biāo)簽選擇：支持His、GST、Flag、MBP等多種標(biāo)簽，便于后續(xù)純化、檢測(cè)與功能研究。
- 多領(lǐng)域應(yīng)用：適用于抗原制備、細(xì)胞與分析、蛋白互作、藥物研發(fā)、酶活性檢測(cè)、ELISA標(biāo)準(zhǔn)品、體內(nèi)外研究、質(zhì)譜與結(jié)構(gòu)分析等廣泛場(chǎng)景。
- 無(wú)動(dòng)物源成分：我們已建立全程無(wú)動(dòng)物副產(chǎn)品接觸的重組蛋白生產(chǎn)線，確保產(chǎn)品的高純凈性與一致性，適用于對(duì)來(lái)源敏感的各類實(shí)驗(yàn)。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì)，或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的重組蛋白，請(qǐng)查看華美生物蛋白定制服務(wù)。
通過(guò)選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
| 產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
|---|---|---|
| Recombinant Pongo abelii 40S ribosomal protein S15 (RPS15) | CSB-YP735826PYX CSB-EP735826PYX CSB-BP735826PYX CSB-MP735826PYX CSB-EP735826PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1 (LINGO1), partial | CSB-YP735827PYX CSB-EP735827PYX CSB-BP735827PYX CSB-MP735827PYX CSB-EP735827PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii SH3-containing GRB2-like protein 3-interacting protein 1 (SGIP1), partial | CSB-YP735828PYX CSB-EP735828PYX CSB-BP735828PYX CSB-MP735828PYX CSB-EP735828PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii Ras-related protein Rap-1b (RAP1B) | CSB-YP735829PYX CSB-EP735829PYX CSB-BP735829PYX CSB-MP735829PYX CSB-EP735829PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii Probable leucine--tRNA ligase, mitochondrial (LARS2) | CSB-YP735830PYX CSB-EP735830PYX CSB-BP735830PYX CSB-MP735830PYX CSB-EP735830PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii UPF0488 protein C8orf33 homolog | CSB-YP735831PYX CSB-EP735831PYX CSB-BP735831PYX CSB-MP735831PYX CSB-EP735831PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii Neuropathy target esterase (PNPLA6), partial | CSB-YP735832PYX CSB-EP735832PYX CSB-BP735832PYX CSB-MP735832PYX CSB-EP735832PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii Histone-lysine N-methyltransferase EZH1 (EZH1), partial | CSB-YP735833PYX CSB-EP735833PYX CSB-BP735833PYX CSB-MP735833PYX CSB-EP735833PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii Enhancer of mRNA-decapping protein 3 (EDC3), partial | CSB-YP735834PYX CSB-EP735834PYX CSB-BP735834PYX CSB-MP735834PYX CSB-EP735834PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii Vacuolar ATPase assembly integral membrane protein VMA21 (VMA21), partial | CSB-YP735835PYX1 CSB-EP735835PYX1 CSB-BP735835PYX1 CSB-MP735835PYX1 CSB-EP735835PYX1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii 55 kDa erythrocyte membrane protein (MPP1) | CSB-YP735836PYX CSB-EP735836PYX CSB-BP735836PYX CSB-MP735836PYX CSB-EP735836PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii Kelch repeat and BTB domain-containing protein 2 (KBTBD2), partial | CSB-YP735837PYX CSB-EP735837PYX CSB-BP735837PYX CSB-MP735837PYX CSB-EP735837PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii Tetraspanin-3 (TSPAN3), partial | CSB-YP735838PYX1 CSB-EP735838PYX1 CSB-BP735838PYX1 CSB-MP735838PYX1 CSB-EP735838PYX1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii HAUS augmin-like complex subunit 2 (HAUS2) | CSB-YP735839PYX CSB-EP735839PYX CSB-BP735839PYX CSB-MP735839PYX CSB-EP735839PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii Anaphase-promoting complex subunit 5 (ANAPC5), partial | CSB-YP735840PYX CSB-EP735840PYX CSB-BP735840PYX CSB-MP735840PYX CSB-EP735840PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii Lanosterol 14-alpha demethylase (CYP51A1), partial | CSB-YP735841PYX1 CSB-EP735841PYX1 CSB-BP735841PYX1 CSB-MP735841PYX1 CSB-EP735841PYX1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii Type 2 lactosamine alpha-2,3-sialyltransferase (ST3GAL6), partial | CSB-YP735842PYX CSB-EP735842PYX CSB-BP735842PYX CSB-MP735842PYX CSB-EP735842PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii Gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), partial | CSB-YP735843PYX CSB-EP735843PYX CSB-BP735843PYX CSB-MP735843PYX CSB-EP735843PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii Myotubularin-related protein 2 (MTMR2), partial | CSB-YP735844PYX CSB-EP735844PYX CSB-BP735844PYX CSB-MP735844PYX CSB-EP735844PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pongo abelii DNA-directed RNA polymerase II subunit GRINL1A (POLR2M) | CSB-YP735845PYX CSB-EP735845PYX CSB-BP735845PYX CSB-MP735845PYX CSB-EP735845PYX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。
第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條：如何選擇合適的表達(dá)載體?
表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過(guò)多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。
根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn)，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022
