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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Probable DNA repair protein ykoV (ykoV) CSB-YP523559BRJ
CSB-EP523559BRJ
CSB-BP523559BRJ
CSB-MP523559BRJ
CSB-EP523559BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative transporter yoaB (yoaB), partial CSB-YP523560BRJ
CSB-EP523560BRJ
CSB-BP523560BRJ
CSB-MP523560BRJ
CSB-EP523560BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative carbonic anhydrase YtiB (ytiB) CSB-YP523561BRJ
CSB-EP523561BRJ
CSB-BP523561BRJ
CSB-MP523561BRJ
CSB-EP523561BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glycine betaine/carnitine/choline transport system permease protein OpuCB (opuCB), partial CSB-YP523562BRJ1
CSB-EP523562BRJ1
CSB-BP523562BRJ1
CSB-MP523562BRJ1
CSB-EP523562BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable tautomerase yolI (yolI) CSB-YP523563BRJ
CSB-EP523563BRJ
CSB-BP523563BRJ
CSB-MP523563BRJ
CSB-EP523563BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yflB (yflB) CSB-YP523564BRJ
CSB-EP523564BRJ
CSB-BP523564BRJ
CSB-MP523564BRJ
CSB-EP523564BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Septation ring formation regulator EzrA (ezrA), partial CSB-YP523565BRJ
CSB-EP523565BRJ
CSB-BP523565BRJ
CSB-MP523565BRJ
CSB-EP523565BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized transcriptional regulatory protein ykoG (ykoG) CSB-YP523566BRJ
CSB-EP523566BRJ
CSB-BP523566BRJ
CSB-MP523566BRJ
CSB-EP523566BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yobT (yobT) CSB-YP523567BRJ
CSB-EP523567BRJ
CSB-BP523567BRJ
CSB-MP523567BRJ
CSB-EP523567BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis N-acetyldiaminopimelate deacetylase (ykuR) CSB-YP523568BRJ
CSB-EP523568BRJ
CSB-BP523568BRJ
CSB-MP523568BRJ
CSB-EP523568BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ytoI (ytoI) CSB-YP523569BRJ
CSB-EP523569BRJ
CSB-BP523569BRJ
CSB-MP523569BRJ
CSB-EP523569BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP523570BRJ
CSB-EP523570BRJ
CSB-BP523570BRJ
CSB-MP523570BRJ
CSB-EP523570BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Response regulator aspartate phosphatase K (rapK) CSB-YP523571BRJ
CSB-EP523571BRJ
CSB-BP523571BRJ
CSB-MP523571BRJ
CSB-EP523571BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yopU (yopU) CSB-YP523572BRJ
CSB-EP523572BRJ
CSB-BP523572BRJ
CSB-MP523572BRJ
CSB-EP523572BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative dipeptidase ytjP (ytjP) CSB-YP523573BRJ
CSB-EP523573BRJ
CSB-BP523573BRJ
CSB-MP523573BRJ
CSB-EP523573BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sensory transduction protein BceR (bceR) CSB-YP523574BRJ
CSB-EP523574BRJ
CSB-BP523574BRJ
CSB-MP523574BRJ
CSB-EP523574BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjaV (yjaV) CSB-YP523575BRJ
CSB-EP523575BRJ
CSB-BP523575BRJ
CSB-MP523575BRJ
CSB-EP523575BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable zinc transport system zinc-binding lipoprotein AdcA (adcA) CSB-YP523576BRJ
CSB-EP523576BRJ
CSB-BP523576BRJ
CSB-MP523576BRJ
CSB-EP523576BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rathayibacter rathayi Sensor protein KdpD (kdpD), partial CSB-YP523577RJU
CSB-EP523577RJU
CSB-BP523577RJU
CSB-MP523577RJU
CSB-EP523577RJU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Guanine/hypoxanthine permease PbuO (pbuO), partial CSB-YP523578BRJ
CSB-EP523578BRJ
CSB-BP523578BRJ
CSB-MP523578BRJ
CSB-EP523578BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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