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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Peptidoglycan endopeptidase LytF (lytF) CSB-YP517446BRJ
CSB-EP517446BRJ
CSB-BP517446BRJ
CSB-MP517446BRJ
CSB-EP517446BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Stress response protein yhaX (yhaX) CSB-YP517447BRJ
CSB-EP517447BRJ
CSB-BP517447BRJ
CSB-MP517447BRJ
CSB-EP517447BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yheF (yheF) CSB-YP517448BRJ
CSB-EP517448BRJ
CSB-BP517448BRJ
CSB-MP517448BRJ
CSB-EP517448BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Na (+)/H (+) antiporter NhaC, partial CSB-YP517449BRJ
CSB-EP517449BRJ
CSB-BP517449BRJ
CSB-MP517449BRJ
CSB-EP517449BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Signal peptidase I V (sipV), partial CSB-YP517450BRJ
CSB-EP517450BRJ
CSB-BP517450BRJ
CSB-MP517450BRJ
CSB-EP517450BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis NTD biosynthesis operon regulator ntdR (ntdR) CSB-YP517451BRJ
CSB-EP517451BRJ
CSB-BP517451BRJ
CSB-MP517451BRJ
CSB-EP517451BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional regulator NsrR (nsrR) CSB-YP517452BRJ
CSB-EP517452BRJ
CSB-BP517452BRJ
CSB-MP517452BRJ
CSB-EP517452BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable anti-sigma-M factor yhdK (yhdK), partial CSB-YP517453BRJ1
CSB-EP517453BRJ1
CSB-BP517453BRJ1
CSB-MP517453BRJ1
CSB-EP517453BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative aspartate aminotransferase YhdR (yhdR) CSB-YP517454BRJ
CSB-EP517454BRJ
CSB-BP517454BRJ
CSB-MP517454BRJ
CSB-EP517454BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MscS family protein YhdY (yhdY), partial CSB-YP517455BRJ1
CSB-EP517455BRJ1
CSB-BP517455BRJ1
CSB-MP517455BRJ1
CSB-EP517455BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 2 (fabHB) CSB-YP517456BRJ
CSB-EP517456BRJ
CSB-BP517456BRJ
CSB-MP517456BRJ
CSB-EP517456BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yhfI (yhfI) CSB-YP517457BRJ
CSB-EP517457BRJ
CSB-BP517457BRJ
CSB-MP517457BRJ
CSB-EP517457BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative quinone oxidoreductase YhfP (yhfP) CSB-YP517458BRJ
CSB-EP517458BRJ
CSB-BP517458BRJ
CSB-MP517458BRJ
CSB-EP517458BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative Rieske 2Fe-2S iron-sulfur protein yhfW (yhfW), partial CSB-YP517459BRJ
CSB-EP517459BRJ
CSB-BP517459BRJ
CSB-MP517459BRJ
CSB-EP517459BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Anti-sigma-ylaC factor ylaD (ylaD), partial CSB-YP517460BRJ1
CSB-EP517460BRJ1
CSB-BP517460BRJ1
CSB-MP517460BRJ1
CSB-EP517460BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized lipoprotein ylaJ (ylaJ) CSB-YP517461BRJ
CSB-EP517461BRJ
CSB-BP517461BRJ
CSB-MP517461BRJ
CSB-EP517461BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Nitrogenase iron protein (nifH) CSB-YP517462DZY
CSB-EP517462DZY
CSB-BP517462DZY
CSB-MP517462DZY
CSB-EP517462DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP517463ELY
CSB-EP517463ELY
CSB-BP517463ELY
CSB-MP517463ELY
CSB-EP517463ELY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Uncharacterized protein HP_1070 (HP_1070) CSB-YP517464HUV
CSB-EP517464HUV
CSB-BP517464HUV
CSB-MP517464HUV
CSB-EP517464HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv1899c/MT1950 (Rv1899c, MT1950) CSB-YP517465MVZ
CSB-EP517465MVZ
CSB-BP517465MVZ
CSB-MP517465MVZ
CSB-EP517465MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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