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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Probable NAD-dependent malic enzyme 3 (malS), partial CSB-YP516484BRJ
CSB-EP516484BRJ
CSB-BP516484BRJ
CSB-MP516484BRJ
CSB-EP516484BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glutamate synthase [NADPH] small chain (gltB) CSB-YP516485BRJ
CSB-EP516485BRJ
CSB-BP516485BRJ
CSB-MP516485BRJ
CSB-EP516485BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ykzD (ykzD) CSB-YP516486BRJ
CSB-EP516486BRJ
CSB-BP516486BRJ
CSB-MP516486BRJ
CSB-EP516486BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Regulatory protein ylbF (ylbF) CSB-YP516487BRJ
CSB-EP516487BRJ
CSB-BP516487BRJ
CSB-MP516487BRJ
CSB-EP516487BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Cryptic phage CTXphi transcriptional repressor rstR (rstR1) CSB-YP516488VEX
CSB-EP516488VEX
CSB-BP516488VEX
CSB-MP516488VEX
CSB-EP516488VEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Calcium-transporting ATPase (yloB), partial CSB-YP516489BRJ
CSB-EP516489BRJ
CSB-BP516489BRJ
CSB-MP516489BRJ
CSB-EP516489BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter yfmI (yfmI), partial CSB-YP516490BRJ
CSB-EP516490BRJ
CSB-BP516490BRJ
CSB-MP516490BRJ
CSB-EP516490BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ylbN (ylbN) CSB-YP516491BRJ
CSB-EP516491BRJ
CSB-BP516491BRJ
CSB-MP516491BRJ
CSB-EP516491BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis N-acetylglucosamine-6-phosphate deacetylase (nagA) CSB-YP516492BRJ
CSB-EP516492BRJ
CSB-BP516492BRJ
CSB-MP516492BRJ
CSB-EP516492BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0111 protein ykaA (ykaA) CSB-YP516493BRJ
CSB-EP516493BRJ
CSB-BP516493BRJ
CSB-MP516493BRJ
CSB-EP516493BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP516494BRJ
CSB-EP516494BRJ
CSB-BP516494BRJ
CSB-MP516494BRJ
CSB-EP516494BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative ATP-dependent helicase yeeB (yeeB), partial CSB-YP516495BRJ
CSB-EP516495BRJ
CSB-BP516495BRJ
CSB-MP516495BRJ
CSB-EP516495BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative NAD (P)H nitroreductase yfkO CSB-YP516496BRJ
CSB-EP516496BRJ
CSB-BP516496BRJ
CSB-MP516496BRJ
CSB-EP516496BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis L-asparaginase 2 (ansZ) CSB-YP516497BRJ
CSB-EP516497BRJ
CSB-BP516497BRJ
CSB-MP516497BRJ
CSB-EP516497BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized peptidase ytmA (ytmA) CSB-YP516498BRJ
CSB-EP516498BRJ
CSB-BP516498BRJ
CSB-MP516498BRJ
CSB-EP516498BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Manganese transport system membrane protein mntD (mntD), partial CSB-YP516499BRJ
CSB-EP516499BRJ
CSB-BP516499BRJ
CSB-MP516499BRJ
CSB-EP516499BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0713 protein yngL (yngL), partial CSB-YP516500BRJ1
CSB-EP516500BRJ1
CSB-BP516500BRJ1
CSB-MP516500BRJ1
CSB-EP516500BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter ATP-binding protein YfmM (yfmM), partial CSB-YP516501BRJ
CSB-EP516501BRJ
CSB-BP516501BRJ
CSB-MP516501BRJ
CSB-EP516501BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis ATP phosphoribosyltransferase (hisG) CSB-YP516502BRJ
CSB-EP516502BRJ
CSB-BP516502BRJ
CSB-MP516502BRJ
CSB-EP516502BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative signal peptide peptidase sppA (sppA), partial CSB-YP516503BRJ
CSB-EP516503BRJ
CSB-BP516503BRJ
CSB-MP516503BRJ
CSB-EP516503BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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