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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus anthracis Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP501459BQG
CSB-EP501459BQG
CSB-BP501459BQG
CSB-MP501459BQG
CSB-EP501459BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Shikimate dehydrogenase (aroE) CSB-YP501460BQG
CSB-EP501460BQG
CSB-BP501460BQG
CSB-MP501460BQG
CSB-EP501460BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP501461BQG
CSB-EP501461BQG
CSB-BP501461BQG
CSB-MP501461BQG
CSB-EP501461BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Uncharacterized methyltransferase BAMEG_4640 (BAMEG_4640) CSB-YP501462BQG
CSB-EP501462BQG
CSB-BP501462BQG
CSB-MP501462BQG
CSB-EP501462BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Transcription elongation factor GreA (greA) CSB-YP501463BQG
CSB-EP501463BQG
CSB-BP501463BQG
CSB-MP501463BQG
CSB-EP501463BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Uridine kinase (udk) CSB-YP501464BQG
CSB-EP501464BQG
CSB-BP501464BQG
CSB-MP501464BQG
CSB-EP501464BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Putative Holliday junction resolvase (BAMEG_4651) CSB-YP501465BQG
CSB-EP501465BQG
CSB-BP501465BQG
CSB-MP501465BQG
CSB-EP501465BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0297 protein BAMEG_4652 (BAMEG_4652) CSB-YP501466BQG
CSB-EP501466BQG
CSB-BP501466BQG
CSB-MP501466BQG
CSB-EP501466BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Aspartate--tRNA ligase (aspS), partial CSB-YP501467BQG
CSB-EP501467BQG
CSB-BP501467BQG
CSB-MP501467BQG
CSB-EP501467BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis D-tyrosyl-tRNA (Tyr) deacylase CSB-YP501468BQG
CSB-EP501468BQG
CSB-BP501468BQG
CSB-MP501468BQG
CSB-EP501468BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Hydroxyethylthiazole kinase (thiM) CSB-YP501469BQG
CSB-EP501469BQG
CSB-BP501469BQG
CSB-MP501469BQG
CSB-EP501469BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Thiamine-phosphate synthase (thiE) CSB-YP501470BQG
CSB-EP501470BQG
CSB-BP501470BQG
CSB-MP501470BQG
CSB-EP501470BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0435 protein BAMEG_0475 (BAMEG_0475) CSB-YP501471BQG
CSB-EP501471BQG
CSB-BP501471BQG
CSB-MP501471BQG
CSB-EP501471BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Metallothiol transferase fosB 2 (fosB2) CSB-YP501472BQG
CSB-EP501472BQG
CSB-BP501472BQG
CSB-MP501472BQG
CSB-EP501472BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 50S ribosomal protein L32 (rpmF) CSB-YP501473BQG
CSB-EP501473BQG
CSB-BP501473BQG
CSB-MP501473BQG
CSB-EP501473BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Uncharacterized N-acetyltransferase BAMEG_0567 (BAMEG_0567) CSB-YP501474BQG
CSB-EP501474BQG
CSB-BP501474BQG
CSB-MP501474BQG
CSB-EP501474BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0747 protein BAMEG_0569 (BAMEG_0569), partial CSB-YP501475BQG
CSB-EP501475BQG
CSB-BP501475BQG
CSB-MP501475BQG
CSB-EP501475BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP501476BQG
CSB-EP501476BQG
CSB-BP501476BQG
CSB-MP501476BQG
CSB-EP501476BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Deoxyribose-phosphate aldolase (deoC) CSB-YP501477BQG
CSB-EP501477BQG
CSB-BP501477BQG
CSB-MP501477BQG
CSB-EP501477BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0176 protein BAMEG_2712 (BAMEG_2712) CSB-YP501478BQG
CSB-EP501478BQG
CSB-BP501478BQG
CSB-MP501478BQG
CSB-EP501478BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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