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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brevibacillus brevis Phosphoglucosamine mutase (glmM) CSB-YP499758BWL
CSB-EP499758BWL
CSB-BP499758BWL
CSB-MP499758BWL
CSB-EP499758BWL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brevibacillus brevis Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP499759BWL
CSB-EP499759BWL
CSB-BP499759BWL
CSB-MP499759BWL
CSB-EP499759BWL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP499760RLK
CSB-EP499760RLK
CSB-BP499760RLK
CSB-MP499760RLK
CSB-EP499760RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP499761RLK
CSB-EP499761RLK
CSB-BP499761RLK
CSB-MP499761RLK
CSB-EP499761RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Acetaldehyde dehydrogenase 1 (RER_07400) CSB-YP499762RLK
CSB-EP499762RLK
CSB-BP499762RLK
CSB-MP499762RLK
CSB-EP499762RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis FMN-dependent NADH-azoreductase (azoR) CSB-YP499763RLK
CSB-EP499763RLK
CSB-BP499763RLK
CSB-MP499763RLK
CSB-EP499763RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis D-inositol 3-phosphate glycosyltransferase (mshA) CSB-YP499764RLK
CSB-EP499764RLK
CSB-BP499764RLK
CSB-MP499764RLK
CSB-EP499764RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 50S ribosomal protein L9 (rplI) CSB-YP499765RLK
CSB-EP499765RLK
CSB-BP499765RLK
CSB-MP499765RLK
CSB-EP499765RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP499766RLK
CSB-EP499766RLK
CSB-BP499766RLK
CSB-MP499766RLK
CSB-EP499766RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 50S ribosomal protein L3 (rplC) CSB-YP499767RLK
CSB-EP499767RLK
CSB-BP499767RLK
CSB-MP499767RLK
CSB-EP499767RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 30S ribosomal protein S17 (rpsQ) CSB-YP499768RLK
CSB-EP499768RLK
CSB-BP499768RLK
CSB-MP499768RLK
CSB-EP499768RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 50S ribosomal protein L15 (rplO) CSB-YP499769RLK
CSB-EP499769RLK
CSB-BP499769RLK
CSB-MP499769RLK
CSB-EP499769RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 50S ribosomal protein L17 (rplQ) CSB-YP499770RLK
CSB-EP499770RLK
CSB-BP499770RLK
CSB-MP499770RLK
CSB-EP499770RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP499771RLK
CSB-EP499771RLK
CSB-BP499771RLK
CSB-MP499771RLK
CSB-EP499771RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP499772RLK
CSB-EP499772RLK
CSB-BP499772RLK
CSB-MP499772RLK
CSB-EP499772RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 2-phospho-L-lactate guanylyltransferase (cofC) CSB-YP499773RLK
CSB-EP499773RLK
CSB-BP499773RLK
CSB-MP499773RLK
CSB-EP499773RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 50S ribosomal protein L19 (rplS) CSB-YP499774RLK
CSB-EP499774RLK
CSB-BP499774RLK
CSB-MP499774RLK
CSB-EP499774RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Proline--tRNA ligase (proS), partial CSB-YP499775RLK
CSB-EP499775RLK
CSB-BP499775RLK
CSB-MP499775RLK
CSB-EP499775RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP499776RLK1
CSB-EP499776RLK1
CSB-BP499776RLK1
CSB-MP499776RLK1
CSB-EP499776RLK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP499777RLK
CSB-EP499777RLK
CSB-BP499777RLK
CSB-MP499777RLK
CSB-EP499777RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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