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重組蛋白

產品名稱 貨號 規格
Recombinant Escherichia coli O81 Fatty acid oxidation complex subunit alpha (fadB) CSB-YP483634EOP
CSB-EP483634EOP
CSB-BP483634EOP
CSB-MP483634EOP
CSB-EP483634EOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O81 ATP synthase gamma chain (atpG) CSB-YP483635EOP
CSB-EP483635EOP
CSB-BP483635EOP
CSB-MP483635EOP
CSB-EP483635EOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O81 Low affinity potassium transport system protein kup (kup), partial CSB-YP483636EOP
CSB-EP483636EOP
CSB-BP483636EOP
CSB-MP483636EOP
CSB-EP483636EOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O81 UPF0761 membrane protein yihY (yihY), partial CSB-YP483637EOP1
CSB-EP483637EOP1
CSB-BP483637EOP1
CSB-MP483637EOP1
CSB-EP483637EOP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O81 Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP483638EOP
CSB-EP483638EOP
CSB-BP483638EOP
CSB-MP483638EOP
CSB-EP483638EOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O81 Glycerol kinase (glpK) CSB-YP483639EOP
CSB-EP483639EOP
CSB-BP483639EOP
CSB-MP483639EOP
CSB-EP483639EOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O81 Deoxyribose-phosphate aldolase (deoC) CSB-YP483640EOP
CSB-EP483640EOP
CSB-BP483640EOP
CSB-MP483640EOP
CSB-EP483640EOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O81 Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP483641EOP
CSB-EP483641EOP
CSB-BP483641EOP
CSB-MP483641EOP
CSB-EP483641EOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Dihydroorotate dehydrogenase (quinone) CSB-YP483642EOG
CSB-EP483642EOG
CSB-BP483642EOG
CSB-MP483642EOG
CSB-EP483642EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Heat shock protein hspQ (hspQ) CSB-YP483643EOG
CSB-EP483643EOG
CSB-BP483643EOG
CSB-MP483643EOG
CSB-EP483643EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Putative aminoacrylate hydrolase RutD (rutD) CSB-YP483644EOG
CSB-EP483644EOG
CSB-BP483644EOG
CSB-MP483644EOG
CSB-EP483644EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 High frequency lysogenization protein HflD (hflD) CSB-YP483645EOG
CSB-EP483645EOG
CSB-BP483645EOG
CSB-MP483645EOG
CSB-EP483645EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Na (+)/H (+) antiporter nhaB, partial CSB-YP483646EOG
CSB-EP483646EOG
CSB-BP483646EOG
CSB-MP483646EOG
CSB-EP483646EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Outer-membrane lipoprotein lolB (lolB) CSB-YP483647EOG
CSB-EP483647EOG
CSB-BP483647EOG
CSB-MP483647EOG
CSB-EP483647EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Probable intracellular septation protein A (yciB), partial CSB-YP483648EOG1
CSB-EP483648EOG1
CSB-BP483648EOG1
CSB-MP483648EOG1
CSB-EP483648EOG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Probable sugar efflux transporter (sotB), partial CSB-YP483649EOG
CSB-EP483649EOG
CSB-BP483649EOG
CSB-MP483649EOG
CSB-EP483649EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Vitamin B12 import system permease protein BtuC (btuC), partial CSB-YP483650EOG
CSB-EP483650EOG
CSB-BP483650EOG
CSB-MP483650EOG
CSB-EP483650EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 NH (3)-dependent NAD (+) synthetase CSB-YP483651EOG
CSB-EP483651EOG
CSB-BP483651EOG
CSB-MP483651EOG
CSB-EP483651EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP483652EOG
CSB-EP483652EOG
CSB-BP483652EOG
CSB-MP483652EOG
CSB-EP483652EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP483653EOG
CSB-EP483653EOG
CSB-BP483653EOG
CSB-MP483653EOG
CSB-EP483653EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.practicalworld.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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