欧美最新另类人妖I国产成人av一区二区三区在线观看I九九热精品视频在线观看I国产精品日韩在线I激情av五月婷婷I日韩精品免费

Your Good Partner in Biology Research

  1. 首頁
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Zaire ebolavirus Transcriptional activator VP30 (VP30)

Recombinant Zaire ebolavirus Transcriptional activator VP30 (VP30)

In Stock
  • 中文名稱：
    Recombinant Zaire ebolavirus Transcriptional activator VP30 (VP30)
  • 品名簡稱：
    Recombinant Zaire ebolavirus VP30 protein
  • 貨號：
    CSB-EP755264ZATa0
  • 說明書：
  • 規格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    VP30
  • Uniprot No.：
  • 別名：
    VP30; Minor nucleoprotein VP30; EbolaVP30; eVP30; Transcription activator VP30
  • 種屬：
    Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    38.0kDa
  • 表達區域：
    1-288aa
  • 氨基酸序列
    MEASYERGRPRAARQHSRDGHDHHVRARSSSRENYRGEYRQSRSASQVRVPTVFHKKRVEPLTVPPAPKDICPTLKKGFLCDSSFCKKDHQLESLTDRELLLLIARKTCGSVEQQLNITAPKDSRLANPTADDFQQEEGPKITLLTLIKTAEHWARQDIRTIEDSKLRALLTLCAVMTRKFSKSQLSLLCETHLRREGLGQDQAEPVLEVYQRLHSDKGGSFEAALWQQWDRQSLIMFITAFLNIALQLPCESSAVVVSGLRTLVPQSDNEEASTNPGTCSWSDEGTP
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    N-terminal 6xHis-tagged
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Multifunctional protein that acts as a viral transcriptional activator. Promotes read-through of an RNA hairpin in the NP open reading frame to enhance viral transcription. Mechanistically, nonphosphorylated VP30 hexamers form a ternary complex with the viral leader RNA. Clamps the RNA template and the complex VP35-polymerase L together, thereby increasing the polymerase affinity for the RNA template to increase transcription initiation despite the presence of RNA secondary structures. Assists also stop-start transcription at gene junctions to promote transcription of downstream genes. Interaction with NP plays a critical role in transcription initiation by recognizing the RNA stem loop. Interaction with host RBBP6 interferes with NP-VP30 interaction and inhibits viral RNA synthesis. Also acts as a suppressor of RNA silencing by interacting with host DICER1 and TARBP2/TRBP.
  • 基因功能參考文獻：
    1. Functional analyses of the eVP30-nucleoprotein interface identify residues that are critical for viral RNA synthesis. Altogether, these results support a model where the eVP30-nucleoprotein interaction plays a critical role in transcription initiation and provides a novel target for the development of antiviral therapy. PMID: 28593988
    2. Comparison of the percentage of disorder (PID) in nucleocapsid proteins VP30 and NP reveals high correlation between nucleocapsid PIDs and the case-fatality rates of Ebola virus . PMID: 26086270
    3. mutations of Thr(143) and Thr(146) of VP30 significantly inhibited EBOV transcription and strongly induced VP30 phosphorylation in the N-terminal Ser residues 29-46, suggesting a novel mechanism of regulation of VP30 phosphorylation. PMID: 24936058
    4. Study shows for the first time the direct interaction of EBOV VP30 with RNA and the importance of the N-terminal region for binding RNA. PMID: 17567691
    5. VP30 is involved in reinitiation of gene transcription and that this activity is affected by mutations at the phosphorylation sites. PMID: 18829754

    顯示更多

    收起更多

  • 亞細胞定位：
    Virion. Host cytoplasm.
  • 蛋白家族：
    Filoviridae minor nucleoprotein VP30 family
  • 數據庫鏈接：

    KEGG: vg:911826



×
生命科學計算平臺