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  4. Recombinant Yersinia pseudotuberculosis serotype I Yop proteins translocation protein M (yscM)

Recombinant Yersinia pseudotuberculosis serotype I Yop proteins translocation protein M (yscM)

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  • 中文名稱：
    Recombinant Yersinia pseudotuberculosis serotype I Yop proteins translocation protein M(yscM)
  • 品名簡稱：
    Recombinant Yersinia pseudotuberculosis serotype I yscM protein
  • 貨號：
    CSB-EP300275YAH
  • 說明書：
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    yscM
  • Uniprot No.：
  • 別名：
    yscM; lcrQ; pYV0089; Yop proteins translocation protein M; Low calcium response locus protein Q
  • 種屬：
    Yersinia pseudotuberculosis serotype I (strain IP32953)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    28.4kDa
  • 表達(dá)區(qū)域：
    1-115aa
  • 氨基酸序列
    MKINTLQSLINQQITQVGHGGQAGRLTETNPLTENSHQISTAEKAFANEVLEHVKNTALSRHDIACLLPRVSNLELKQGKAGEVIVTGLRTEQLSLSDAKLLLEAAMRQDTAADG
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-SUMO-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    Recombinant Y.pseudotuberculosis serotype I yscM protein is an E.coli-expressed Full Length?protein. Molecularly, this yscM protein is characterized by N-terminal 6xHis-SUMO tag, internal Y.pseudotuberculosis serotype I yscM?DNA fragment (1-115aa). Standard methods for recombinant yscM?protein expression comprise transfecting cells with DNA vectors that consist of specific templates and then cultures cells to translate and transcribe the yscM?protein production process. Typically, these cells are lysed to extract expressed proteins for more purification. The purity of this recombinant yscM protein?is 90%+ measured by SDS-PAGE.

    yscM, also called LcrQ, is a regulatory protein specific to Yersinia. LcrQ acts as an anti-activator that causes feedback inhibition for Ysc-Yop type III secretion system (T3SS) gene expression. LcrQ is secreted outside of cells by the secretory machinery under low calcium conditions at 37 °C with ligation to the chaperone protein SycH, easing the inhibitory effect of LcrQ and leading to the activation of the Ysc-Yop T3SS. LcrQ-SycH interaction also promotes the T3SS of LcrQ to the outside environment, further increasing Yop synthesis and secretion.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Belongs to an operon involved in the translocation of Yop proteins across the bacterial membranes or in the specific control of this function.
  • 數(shù)據(jù)庫鏈接：


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