Recombinant Schizosaccharomyces pombe DASH complex subunit dam1 (dam1)

中文名稱：

Recombinant Schizosaccharomyces pombe DASH complex subunit dam1(dam1)
貨號：

CSB-YP888041SXV
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Schizosaccharomyces pombe DASH complex subunit dam1(dam1)
貨號：

CSB-EP888041SXV
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Schizosaccharomyces pombe DASH complex subunit dam1(dam1)
貨號：

CSB-EP888041SXV-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Schizosaccharomyces pombe DASH complex subunit dam1(dam1)
貨號：

CSB-BP888041SXV
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Schizosaccharomyces pombe DASH complex subunit dam1(dam1)
貨號：

CSB-MP888041SXV
規(guī)格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

dam1
Uniprot No.：

Q9HDZ6
別名：

dam1; SPAC589.08cDASH complex subunit dam1; Outer kinetochore protein dam1
種屬：

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
蛋白長度：

full length protein
表達區(qū)域：

1-155
氨基酸序列

MEKYQKATQN PLENVDNVKI ESENAIPSNL QAFTKSLAVL DDNVSEFRKR MNHLSATKQI LDNFNESFSS FLYGLQINAF CVDYENAPLS ESFLLQAKKD QFKATLMTRT GHSISDPPYD GGVISHDPNF ATADETFATN DTSFIERPET YSASR
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore.
基因功能參考文獻：
1. Fndings suggest that patches, instead of rings, are the physiologically functional forms of Dam1. PMID: 20624975
2. These data provide the first direct evidence that the Dam1/DASH complex can couple the force generated by microtubule depolymerisation to direct chromosome movement in vivo. PMID: 17881496
3. Sister kinetochore recapture in fission yeast occurs by two distinct mechanisms, both requiring Dam1 and Klp2. PMID: 18256284
亞細胞定位：

Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Note=Associates with the mitotic spindle and the kinetochore. Kinetochore association occurs only during mitosis.
蛋白家族：

DASH complex DAM1 family
數據庫鏈接：

KEGG: spo:SPAC589.08c

STRING: 4896.SPAC589.08c.1