Recombinant Salmonella typhimurium Secreted effector protein sifA (sifA)

中文名稱：

鼠傷寒沙門(mén)菌sifA重組蛋白
貨號(hào)：

CSB-YP684910SXB
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

鼠傷寒沙門(mén)菌sifA重組蛋白
貨號(hào)：

CSB-EP684910SXB
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

鼠傷寒沙門(mén)菌sifA重組蛋白
貨號(hào)：

CSB-EP684910SXB-B
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

鼠傷寒沙門(mén)菌sifA重組蛋白
貨號(hào)：

CSB-BP684910SXB
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

鼠傷寒沙門(mén)菌sifA重組蛋白
貨號(hào)：

CSB-MP684910SXB
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

sifA
Uniprot No.：

Q56061
別名：

sifA; STM1224; Secreted effector protein SifA
種屬：

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-336
氨基酸序列

MPITIGNGFL KSEILTNSPR NTKEAWWKVL WEKIKDFFFS TGKAKADRCL HEMLFAERAP TRERLTEIFF ELKELACASQ RDRFQVHNPH ENDATIILRI MDQNEENELL RITQNTDTFS CEVMGNLYFL MKDRPDILKS HPQMTAMIKR RYSEIVDYPL PSTLCLNPAG APILSVPLDN IEGYLYTELR KGHLDGWKAQ EKATYLAAKI QSGIEKTTRI LHHANISEST QQNAFLETMA MCGLKQLEIP PPHTHIPIEK MVKEVLLADK TFQAFLVTDP STSQSMLAEI VEAISDQVFH AIFRIDPQAI QKMAEEQLTT LHVRSEQQSG CLCCFL
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is required for endosomal tubulation and formation of Salmonella-induced filaments (Sifs), which are filamentous structures containing lysosomal membrane glycoproteins within epithelial cells. Sif formation is concomitant with intracellular bacterial replication.
基因功能參考文獻(xiàn)：
1. We used a model of SKIP knockout mice to show that this protein mediates the host susceptibility to salmonellosis and to establish that SifA also contributes to Salmonella virulence independently of its interaction with SKIP PMID: 26268777
2. Authors show that Pleckstrin homology domain-containing protein family member 1 (PLEKHM1), a lysosomal adaptor, is targeted by Salmonella through direct interaction with SifA. PMID: 25500191
3. Data shoe that the N-terminal domain of SifA is sufficient to interact with the pleckstrin homology domain of SKIP, forming a 1:1 complex with a micromolar dissociation constant. PMID: 19801640
4. S. Typhimurium sifA was shown to escape the SCVs (S.typhimurium containing vacuoles) into the cytosol of infected dendritic cells (DCs), and was more efficient than S. Typhimurium aroC as a gene delivery vector to DCs. PMID: 15469435
5. Salmonella typhimurium effector protein SifA is modified by host cell prenylation and S-acylation machinery PMID: 15710609
6. A dynamic process of kinesin recruitment in Salmonella-infected cells is down-regulated by the SifA-mediated recruitment of SKIP on membranes PMID: 15905402
7. SifA, SKIP, SseJ, and RhoA family GTPases cooperatively promote host membrane tubulation. PMID: 18996344
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亞細(xì)胞定位：

Secreted. Host cytoplasm. Host cell membrane. Note=Secreted via type III secretion system 2 (SPI-2 TTSS), and delivered into the host cytoplasm. It associates with membranes and colocalizes with microtubules along the length of Sifs in infected cells.
蛋白家族：

Sif family
數(shù)據(jù)庫(kù)鏈接：

KEGG: stm:STM1224

STRING: 99287.STM1224