Recombinant Rat ATP-dependent RNA helicase DDX25 (Ddx25)

中文名稱：

Recombinant Rat ATP-dependent RNA helicase DDX25(Ddx25)
貨號：

CSB-YP889879RA
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Rat ATP-dependent RNA helicase DDX25(Ddx25)
貨號：

CSB-EP889879RA
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Rat ATP-dependent RNA helicase DDX25(Ddx25)
貨號：

CSB-EP889879RA-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Rat ATP-dependent RNA helicase DDX25(Ddx25)
貨號：

CSB-BP889879RA
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Rat ATP-dependent RNA helicase DDX25(Ddx25)
貨號：

CSB-MP889879RA
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Ddx25
Uniprot No.：

Q9QY16
別名：

Ddx25; GrthATP-dependent RNA helicase DDX25; EC 3.6.4.13; DEAD box protein 25; Gonadotropin-regulated testicular RNA helicase
種屬：

Rattus norvegicus (Rat)
蛋白長度：

full length protein
表達區域：

1-483
氨基酸序列

MASLLWGGDA GAAESERLNS HFSNLVHPRK NLRGIRSTTV PNIDGSLNTE EDDDEDDVVD LAANSLLNKL IRQSLVESSH RVEVLQKDPS SPLYSVKTFE ELRLKEELLK GIYAMGFNRP SKIQEMALPM MLAHPPQNLI AQSQSGTGKT AAFVLAMLNR VNALELFPQC LCLAPTYELA LQTGRVVERM GKFCVDVEVM YAIRGNRIPR GTDVTKQIVI GTPGTVLDWC FKRKLIDLTK IRVFVLDEAD VMIDTQGFSD QSIRIQRALP SECQMLLFSA TFEDSVWQFA ERIIPDPNVI KLRKEELTLN NIRQYYVLCE NRKDKYQALC NIYGGITIGQ AIIFCQTRRN AKWLTVEMMQ DGHQVSLLSG ELTVEQRASI IQRFRDGKEK VLITTNVCAR GIDVKQVTIV VNFDLPVNQS EEPDYETYLH RIGRTGRFGK KGLAFNMIEV DKLPLLMKIQ DHFNSSIKQL DPEDMDEIEK IEY
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development.
基因功能參考文獻：
1. The results suggest that DDX25 functions in meiotic cells,intermitochondrial cement,loose aggregates of particles,chromatoid bodies,late chromatoid bodies,satellite bodies,granulated bodies,mitochondria-associated granules and reticulated bodies. PMID: 22038044
2. studies on GRTH [proide insights into the intrinsic requirements of spermatogenesis and open new avenues for studies on regulating spermatogenesis, infertility and male contraception PMID: 21691948
3. expression is regulated by gonadotropin/androgen at the translational level PMID: 12734186
4. The results of this study indicate that the mammalian GRTH/Ddx25 gene is a multifunctional RNA helicase that is an essential regulator of sperm maturation. PMID: 16968703
亞細胞定位：

Cytoplasm. Nucleus.
蛋白家族：

DEAD box helicase family
組織特異性：

Isoform 1 is expressed in germ cells. Isoform 2 is highly expressed in Leydig cells and weakly expressed in the pituitary and hypothalamus. Isoform 3 is weakly expressed only in germ cells.
數據庫鏈接：

KEGG: rno:58856

STRING: 10116.ENSRNOP00000017307

UniGene: Rn.224821