E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產品提供形式:
Lyophilized powder
Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet :
Please contact us to get it.
產品評價
靶點詳情
功能:
Mediates the Na(+)-independent uptake of organic anions such as taurochlate, bromosulfophthalein and steroid conjugates such as estrone-3-sulfate, 17-beta-glucuronosyl estradiol, dehydroepiandrosterone sulfate and prostaglandin E2.
基因功能參考文獻:
Experiments testing the cellular uptake of androgens suggest that testosterone is an excellent substrate of OATP1B3 and cells expressing a doxycycline-inducible SLCO1B3 construct had greater uptake of a clinically relevant concentration of 3H-testosterone . When compared with Slco1b2 (-/-) mice, Slco1b2 (-/-)/hSLCO1B3 knockins had greater greater hepatic uptakeand lower plasma exposure to 3H-testosterone PMID: 28389619
Protection against phalloidin hepatotoxicity by oleanolic acid involves activation of Nrf2 and suppression of Oatp1b2. PMID: 25280775
the combination of NASH-associated decrease in compensatory Oatp transporters and Oatp1b2 genetic loss caused a synergistic increase in pravastatin plasma and tissue concentrations in kidney and muscle PMID: 24613363
These findings showed that deficiency of Oatp1b transporters alters the absorption, distribution, and elimination of hydroxyurea. PMID: 23986199
Oatp1c1 deficiency has an effect on thyroid hormone metabolism and action in the mouse brain PMID: 22294745
This study indicates that Oatp1b2 has a major role in the hepatic uptake of unconjugated bile acids. PMID: 20949553
Cloning of the full coding region of oatp1b2 revealed the presence of two novel splice variants. Interestingly, these splice variants were significantly expressed in organs such as the kidney, but much less in liver. PMID: 19400585
Review summarizes the evidence for the important role of mouse liver-specific organic anion transporting polypeptide Oatpb2 in drug deposition and hepatotoxicity. PMID: 18492796
Report critical role of PPAR-alpha in perfluorooctanoic acid- and perfluorodecanoic acid-induced downregulation of Oatp1b2 uptake transporter in mouse liver. PMID: 18703564
In the liver, histone H3 in the proximal promoter of Oatp1b2 is hyperacetylated, whereas acetylation in the kidney and the cerebrum is minimal. PMID: 19047482