Recombinant Mouse Phosphatase and actin regulator 4 (Phactr4)

中文名稱：

小鼠Phactr4重組蛋白
貨號：

CSB-YP017883MO
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Phactr4重組蛋白
貨號：

CSB-EP017883MO
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Phactr4重組蛋白
貨號：

CSB-EP017883MO-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Phactr4重組蛋白
貨號：

CSB-BP017883MO
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Phactr4重組蛋白
貨號：

CSB-MP017883MO
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Phactr4
Uniprot No.：

Q501J7
別名：

Phactr4; Kiaa4120Phosphatase and actin regulator 4; Protein Humpty dumpty; Humdy
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-694
氨基酸序列

MEDPSEEAEQ PSGDPGMGMD SVEAGDTTPP TKRKSKFSAL GKIFKPWKWR KKKSSDKFKE TSEVLERKIS MRKPREELVK RGVLLEDPEQ DGEDSGKLSH AALKNGHTTP IGSARSSSPV LVEEEPERSL RNLTPEEESK KRLGSTGSQP NSEAEPGPEH APKQPLLPPK RPLSSSCEAK EVPAGSTARS VSSTSGSTTV TSAATTAATD MTKTVKSFVG PTPAPAPAPR TLPAAPASAN TAATTTAPAK QPPIPPPKPA QRNSNPIIAE LSQAMNSGTV LSKPSPPLPP KRGIPSTSIP SLEPAASFTT KTANDQREKT VSLCLEPPLI IPPSSPSPPL PTHIPPEPPR SPLVPAKTFQ IVPEVEFSSS SDLFQDISQQ EDQKTEVPKK IQDQSFGESH IPSRLPPLPL HIRIQQALTS PLPVTPPLEG THRAHSLLFE NSDSFSEDTG TLGRTRSLPI TIEMLKVPDD EEEEQTCPFV EDVTSTSATP SLPLCLREEE KESDSDSEGP IKYRDEEEDD DDDESHQSAL ANRVKRKDTL AMKLSSRPSE PETNLNSWPR KSKEEWNEIR HQIGNTLIRR LSQRPTAEEL EQRNILQPKN EADRQAEKRE IKRRLTRKLS QRPTVAELLA RKILRFNEYV EVTDAHDYDR RADKPWTKLT PADKAAIRKE LNEFKSSEME VHVDSKHFTR YHRP
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Regulator of protein phosphatase 1 (PP1) required for neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. Acts as an activator of PP1 by interacting with PPP1CA and preventing phosphorylation of PPP1CA at 'Thr-320'. During neural tube closure, localizes to the ventral neural tube and activates PP1, leading to down-regulate cell proliferation within cranial neural tissue and the neural retina. Also acts as a regulator of migration of enteric neural crest cells (ENCCs) by activating PP1, leading to dephosphorylation and subsequent activation of cofilin (COF1 or COF2) and repression of the integrin signaling through the RHO/ROCK pathway.
基因功能參考文獻：
1. The Phactr4 forms functional associations with intermediate filament networks in adult neural stem cells. PMID: 24748504
2. Data discloses the central role of Phactr4 in a feedback loop, where actin monomers regulate their own number via the activation of a key regulator of actin dynamics. PMID: 23203801
3. This study demonistrated that the mRNA expression of phactr4 was progressively increased in the injury penumbra. PMID: 22766235
4. hactr4 negatively regulates integrin signaling through the RHO/ROCK pathway and coordinates protein phosphatase 1 (PP1) with cofilin activity to regulate cytoskeletal dynamics PMID: 22215812
5. Phactr4 is critical for the spatially and temporally regulated transition in proliferation through differential regulation of PP1 and the cell cycle during neurulation and eye development PMID: 17609112
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亞細胞定位：

Cytoplasm. Cell projection, lamellipodium.
蛋白家族：

Phosphatase and actin regulator family
數據庫鏈接：

KEGG: mmu:100169

STRING: 10090.ENSMUSP00000099628

UniGene: Mm.158361