Recombinant Mouse Nuclear distribution protein nudE homolog 1 (Nde1)

中文名稱：

小鼠Nde1重組蛋白
貨號：

CSB-YP887476MO
規(guī)格：
來源：

Yeast
其他：

中文名稱：

小鼠Nde1重組蛋白
貨號：

CSB-EP887476MO
規(guī)格：
來源：

E.coli
其他：

中文名稱：

小鼠Nde1重組蛋白
貨號：

CSB-EP887476MO-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Nde1重組蛋白
貨號：

CSB-BP887476MO
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Nde1重組蛋白
貨號：

CSB-MP887476MO
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Nde1
Uniprot No.：

Q9CZA6
別名：

Nde1; Nude; Nuclear distribution protein nudE homolog 1; NudE; mNudE
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-344
氨基酸序列

MEDSGKTFES EEEETNYWRD LAMTYKQRAE NTQEELREFQ EGSREYEAEL EAQLQQIETR NRDLLSENNR LRMELESVKE KFEMQHSEGY RQISALEDDL AQTKAIKDQL QKYIRELEQA NDDLERAKRA TIMSLEDFEQ RLNQAIERNA FLESELDEKE NLLESVQRLK DEARDLRQEL AVQQKQDKPR TPMPGSGQAK RTDMAVQATG SVPSTPVAHR GPSSGLNTPG MFRRGLDSST SGTPLTPAAR ISALNIVGDL LRKVGALESK LASCRNFMYD QSPSRTSGPA SGRGTKNRDG VDRRPGSTSV GDKGSGKRLE FGKPASEPAS PALPSAQGVV KLLL
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.
基因功能參考文獻(xiàn)：
1. Findings suggest that cell cycle scaffold protein NDE1-mediated heterochromatin replication is indispensible for neuronal differentiation, and that the loss of NDE1 function may lead to genomic neurological disorders. PMID: 25245017
2. study provided comprehensive documentation of the expression patterns of Nde1 and Ndel1 in cultured cells as well as in mouse and human brains, and also highlighting that dosage effects of these two proteins might contribute to some cases of mental disorder PMID: 24785679
3. Nde1 and Lis1 regulate central nervous system development by interpreting midline signals differentially. PMID: 23673330
4. A three-dimensional regulation of radial glial cells' cytoarchitecture by the Lis1-Nde1-DGC complex determines the number and spatial organization of cortical neurons as well as the size and shape of the cerebral cortex. PMID: 22028625
5. Mutually exclusive cytoplasmic dynein regulation by NudE-Lis1 and dynactin. PMID: 21911489
6. NDE1 deficiency causes both a severe failure of neurogenesis and a deficiency in cortical lamination PMID: 21529752
7. These data suggest that Nde1 is an integral component of a network coordinating ciliary length with cell cycle progression. PMID: 21394081
8. Nde1 is essential for centrosome duplication and mitotic spindle assembly. PMID: 15473967
9. Lis1-Nde1 complex is essential for radial glial neuroepithelial progenitors self renewal during the early phase of corticogenesis. PMID: 18469343
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亞細(xì)胞定位：

Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Cleavage furrow. Note=Localizes to the interphase and S phase centrosome. During mitosis, partially associated with the mitotic spindle. Concentrates at the plus ends of microtubules coincident with kinetochores in metaphase and anaphase in a CENPF-dependent manner. Also localizes to the cleavage furrow during cytokinesis.
蛋白家族：

NudE family
組織特異性：

Highly expressed in ovary. Also expressed in brain, heart, kidney, large intestine, liver, lung, small intestine and testis.
數(shù)據(jù)庫鏈接：

KEGG: mmu:67203

STRING: 10090.ENSMUSP00000023359

UniGene: Mm.24105