Recombinant Mouse Myelin gene regulatory factor (MRF), partial

中文名稱：

小鼠Myrf重組蛋白
貨號：

CSB-YP668887MO
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Myrf重組蛋白
貨號：

CSB-EP668887MO
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Myrf重組蛋白
貨號：

CSB-EP668887MO-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Myrf重組蛋白
貨號：

CSB-BP668887MO
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Myrf重組蛋白
貨號：

CSB-MP668887MO
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Myrf
Uniprot No.：

Q3UR85
別名：

Myrf; Gm1804; Gm98; Mrf; Myelin regulatory factor; EC 3.4.-.-; Myelin gene regulatory factor) [Cleaved into: Myelin regulatory factor; N-terminal; Myelin regulatory factor; C-terminal]
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Constitutes a precursor of the transcription factor. Mediates the autocatalytic cleavage that releases the Myelin regulatory factor, N-terminal component that specifically activates transcription of central nervous system (CNS) myelin genes.; Membrane-bound part that has no transcription factor activity and remains attached to the endoplasmic reticulum membrane following cleavage.; Transcription factor that specifically activates expression of myelin genes such as MBP, MOG, MAG, DUSP15 and PLP1 during oligodendrocyte (OL) maturation, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Specifically recognizes and binds DNA sequence 5'-CTGGYAC-3' in the regulatory regions of myelin-specific genes and directly activates their expression. Not only required during oligodendrocyte differentiation but is also required on an ongoing basis for the maintenance of expression of myelin genes and for the maintenance of a mature, viable oligodendrocyte phenotype.
基因功能參考文獻：
1. results thus suggest that Fbxw7 controls the transcription of MyRF target genes in various tissues through regulation of MyRF protein stability in a manner dependent on MyRF phosphorylation by GSK-3. PMID: 29472293
2. Purification, crystallization and X-ray analysis of DNA-binding domain of MRF has been reported. PMID: 28695847
3. molecular mechanisms underlying the homo-trimerization of Myrf N-terminal fragments and the homo-trimeric DNA binding of Myrf N-terminal fragments PMID: 28160598
4. mutant huntingtin binds abnormally to MYRF and affects its transcription activity PMID: 25789755
5. Here we identify Myrf as an oligodendrocyte-specific target of Sox10 and map a Sox10 responsive enhancer to an evolutionarily conserved element in intron 1 of the Myrf gene PMID: 24204311
6. Expression of MRF within the adult central nervous system (CNS) is critical to maintain oligodendrocyte identity and integrity of CNS myelin. PMID: 22956843
7. NPC1 protein was expressed in oligodendrocytes and the amount of myelin protein was generally decreased in all parts of the brain and spinal cord in NPC1-mutant mice PMID: 21938520
8. Study identifies gene model 98, which we have named myelin gene regulatory factor (MRF), as a transcriptional regulator required for CNS myelination. PMID: 19596243
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亞細胞定位：

[Myelin regulatory factor]: Endoplasmic reticulum membrane; Single-pass membrane protein.; [Myelin regulatory factor, N-terminal]: Nucleus. Cytoplasm.; [Myelin regulatory factor, C-terminal]: Endoplasmic reticulum membrane; Single-pass membrane protein.
蛋白家族：

MRF family
組織特異性：

Specifically expressed by postmitotic oligodendrocytes in the CNS. Not detected in the peripheral nervous system (PNS).
數據庫鏈接：

KEGG: mmu:225908

STRING: 10090.ENSMUSP00000085329

UniGene: Mm.329716