E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產(chǎn)品提供形式:
Lyophilized powder
Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復(fù)溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲(chǔ)存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項(xiàng):
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet :
Please contact us to get it.
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情
功能:
Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors. Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway.
基因功能參考文獻(xiàn):
LGI1 is expressed at the axonal initial segment and regulates action potential firing by setting the density of the axonal Kv1.1 channels. PMID: 28673977
LGI1 has a role in regulating cortical development, which is increasingly becoming recognized as one of the causes of idiopathic epilepsy. PMID: 25346110
LGI1 and ADAM22 form an essential synaptic organizing complex that coordinates the maturation of excitatory synapses by regulating the functional incorporation of PSD-95 PMID: 26178195
Review article demonstrating that LGI1 may be an essential player in the development of the brain PMID: 25471260
Glutamatergic neuron-targeted loss of Lgi1 causes a severe epileptic phenotype; Lgi1 displays an essential role in brain during the whole life. PMID: 25234641
Lgi1 contains nuclear localization signal that mediates Lgi1 localization to cytoplasm and nucleus in CNS embryonic neurons. PMID: 22612501
This study demonistrated that LGI1 plays a critical role in both dendritic and axonal pruning, we identify a novel molecular pathway in synapse and circuit remodeling. PMID: 22262888
Lgi1 may have a role in establishing normal brain architecture and neuronal functions during brain development suggesting that it may be involved in neurogenesis and neuronal plasticity PMID: 21569517
LGI1(+/-) and LGI1(-/-) mice may provide useful models for lateral temporal lobe epilepsy, and more generally idiopathic focal epilepsy. PMID: 20659958
in the brain of C57BL/6J adult mice by in situ hybridization. We found that the LGI1 transcript is mainly expressed in the dentate gyrus and CA3 field of the hippocampus. PMID: 19833108
Reduce levels of secreted LGI1 is associated with autosomal dominant partial epilepsy with auditory features PMID: 15857855
Two protein isoforms encoded by LGI1/epitempin are differentially expressed in mouse brain: the 60-kDa form is detected in the microsomal fraction, while the 65-kDa product is found in the cytosolic fraction. PMID: 16787412
This study was the first to determine a specific profile of Lgi1 gene expression during central nervous system development, which suggests a possible inhibitory function in latter stages of development. PMID: 18563303
study identified LGI1 as a specific binding partner of ADAM22 protein from mouse brain, and demonstrated the specific interaction between LGI1 and ADAM22 PMID: 18974846
The normal postnatal maturation of presynaptric and postsynaptic functions was arrested by the 835delC mutant Lgi1. PMID: 19701204
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亞細(xì)胞定位:
Secreted. Cell junction, synapse. Note=Isoform 1 and isoform 2 are detected in the cytosolic and microsomal fractions respectively.
組織特異性:
Brain. Highly expressed in the dentate gyrus and CA3 field of the hippocampus.