Recombinant Mouse Kelch-like protein 22 (Klhl22)

中文名稱：

小鼠Klhl22重組蛋白
貨號(hào)：

CSB-YP860368MO
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

小鼠Klhl22重組蛋白
貨號(hào)：

CSB-EP860368MO
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

小鼠Klhl22重組蛋白
貨號(hào)：

CSB-EP860368MO-B
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Klhl22重組蛋白
貨號(hào)：

CSB-BP860368MO
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

小鼠Klhl22重組蛋白
貨號(hào)：

CSB-MP860368MO
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Klhl22
Uniprot No.：

Q99JN2
別名：

Klhl22; Kelch-like protein 22
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Full Length of Mature Protein
表達(dá)區(qū)域：

2-634
氨基酸序列

AEEQDFAQL CRLPTQPSHS HCVNNTYRST QHSQALLRGL LALRDSGILF DVVLVVEGKH IEAHRILLAA SCDYFRGMFA GGLKEMEQEE VLIHGVSYNA MCQILHFIYT SELELSLSNV QETLVAACQL QIPEIIHFCC DFLMSWVDEE NILDVYRLAD LFDLNHLTQQ LDTYILKNFV AFSRTDKYRQ LPLEKVYSLL SSNRLEVSCE TEVYEGALLY HYSLEQVQAD QISLNEPPKL LETVRFPLME AEVLQRLHDK LGPSPLRDTV ASALMYHRNE ILQPSLQGPQ TELRSDFQCV VGFGGIHSTP STILSDQAKY LNPLLGEWKH FTASLAPRMS NQGIAVLNNF VYLIGGDNNV QGFRAESRCW RYDPRHNRWF QIQSLQQEHA DLCVCVVGKY IYAVAGRDYH NDLSAVERYD PATNSWDYVA PLKKEVYAHA GTTLQGKMYI TCGRRGEDYL KETHCYDPGS NTWHTLADGP VRRAWHGMAA LLDKLFVIGG SNNDAGYRRD VHQVACYSCT SRQWSSVCPL PAGHGEPGIA VLDSRIYVLG GRSHNRGSRT GYVHIYDMEK DCWEEGPQLN NSISGLAACV LTLPRSLLHE QPRGTPNRSQ ADADFASEVM SVSDWEEFDN SSED
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation. The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway. It is therefore an amino acid-dependent activator within the amino acid-sensing branch of the TORC1 pathway, indirectly regulating different cellular processes including cell growth and autophagy.
亞細(xì)胞定位：

Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Nucleus. Lysosome.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:224023

STRING: 10090.ENSMUSP00000112412

UniGene: Mm.282807