Recombinant Mouse KH domain-containing, RNA-binding, signal transduction-associated protein 3 (Khdrbs3)

中文名稱：

小鼠Khdrbs3重組蛋白
貨號(hào)：

CSB-YP890109MO
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

小鼠Khdrbs3重組蛋白
貨號(hào)：

CSB-EP890109MO
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

小鼠Khdrbs3重組蛋白
貨號(hào)：

CSB-EP890109MO-B
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Khdrbs3重組蛋白
貨號(hào)：

CSB-BP890109MO
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

小鼠Khdrbs3重組蛋白
貨號(hào)：

CSB-MP890109MO
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Khdrbs3
Uniprot No.：

Q9R226
別名：

Khdrbs3; Salp; Slm2; KH domain-containing; RNA-binding; signal transduction-associated protein 3; RNA-binding protein Etoile; Sam68-like mammalian protein 2; SLM-2
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-346
氨基酸序列

MEEKYLPELM AEKDSLDPSF THALRLVNRE IEKFQKGEGK EEEKYIDVVI NKNMKLGQKV LIPVKQFPKF NFVGKLLGPR GNSLKRLQEE TLTKMSILGK GSMRDKAKEE ELRKSGEAKY FHLNDDLHVL IEVFAPPAEA YARMGHALEE IKKFLIPDYN DEIRQAQLQE LTYLNGGSEN ADVPVVRGKS TLRTRGVTTP AITRGRGGVT ARPVAVGVPR GTPTPRGVLS TRGPVSRGRG LLTPRARGVP PTGYRPPPPP PTQETYGEYD YDDGYGTAYD EQSYDSYDNS YSTPAQSAAD YYDYGHGLSE DAYDSYGQEE WTNSRHKAPS ARTAKGVYRD QPYGRY
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds preferentially to the 5'-[AU]UAAA-3' motif in vitro. Binds optimally to RNA containing 5'-[AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A). RNA-binding abilities are down-regulated by tyrosine kinase PTK6. Involved in splice site selection of vascular endothelial growth factor. In vitro regulates CD44 alternative splicing by direct binding to purine-rich exonic enhancer. Can regulate alternative splicing of neurexins NRXN1-3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners such as neuroligins and LRRTM family members. High concentrations in forebrain structures block splicing inclusion of NRXN1-3 AS4 exons while low concentrations favor their inclusion. Targeted, cell-type specific splicing regulation of NRXN1 at AS4 is involved in neuronal glutamatergic synapse function and plasticity and is linked to behavioral aspects. Regulates expression of KHDRBS2/SLIM-1 in defined neuron populations in the hippocampus by modifying its alternative splicing resulting in a transcript predicted to undergo nonsense-mediated decay. Can bind FABP9 mRNA. May play a role as a negative regulator of cell growth. Inhibits cell proliferation.
基因功能參考文獻(xiàn)：
1. Our data reveal a pathway of SLM2 homeostatic auto-regulation controlling brain network activity and behavior PMID: 28009295
2. (Nrxn) is a critical element in selective trans-synaptic interactions. This study evaluated the synaptogenic receptor activity of NL1/2/3 isoforms in a neuron-fibroblast co-culture system, in which the Nrxn AS4 segments are manipulated using SLM2, a selective and dominant regulator of AS4 splicing. PMID: 28939043
3. this study found that the RNA-binding protein SLM2 is essential for functional specification of glutamatergic synapses in the mouse hippocampus. PMID: 27174676
4. Sam68 and T-STAR could regulate alternative splicing of some pre-mRNAs by bringing two distant UAA motifs into proximity and looping out regions of the pre-mRNA. PMID: 26758068
5. A hierarchical, SLM2-dependent mechanism in hippocampus was identified for cell type-specific expression of neuronal splicing regulators. PMID: 25505328
6. 10 target mRNA substrates from testis extract are bound by T-STAR. PMID: 19916944
7. expression of active BRK/Sik results in increased SLM-1 and SLM-2 phosphorylation which has functional relevance and leads to inhibition of their RNA-binding PMID: 15471878
顯示更多

收起更多
亞細(xì)胞定位：

Nucleus. Note=Localized in a compartment adjacent to the nucleolus, but distinct from the peri-nucleolar one.
蛋白家族：

KHDRBS family
組織特異性：

Highly expressed in testis and brain. In adult cerebellum expressed predominantly in internal granular layer interneurons and in hippocampus is exclusively expressed in CA neurons; expression is restricted to neuronal subpopulations largely non-overlappin
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:13992

STRING: 10090.ENSMUSP00000022954

UniGene: Mm.17964