Recombinant Mouse Homeobox protein Nkx-6.1 (Nkx6-1)

中文名稱：

小鼠Nkx6-1重組蛋白
貨號(hào)：

CSB-YP860820MO
規(guī)格：
來源：

Yeast
其他：

中文名稱：

小鼠Nkx6-1重組蛋白
貨號(hào)：

CSB-EP860820MO
規(guī)格：
來源：

E.coli
其他：

中文名稱：

小鼠Nkx6-1重組蛋白
貨號(hào)：

CSB-EP860820MO-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Nkx6-1重組蛋白
貨號(hào)：

CSB-BP860820MO
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Nkx6-1重組蛋白
貨號(hào)：

CSB-MP860820MO
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Nkx6-1
Uniprot No.：

Q99MA9
別名：

Nkx6-1; Nkx6.1; Nkx6a; Homeobox protein Nkx-6.1; Homeobox protein NK-6 homolog A
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-365
氨基酸序列

MLAVGAMEGP RQSAFLLSSP PLAALHSMAE MKTPLYPAAY PPLPTGPPSS SSSSSSSSSP SPPLGSHNPG GLKPPAAGGL SSLGSPPQQL SAATPHGIND ILSRPSMPVA SGAALPSASP SGSSSSSSSS ASATSASAAA AAAAAAAAAA ASSPAGLLAG LPRFSSLSPP PPPPGLYFSP SAAAVAAVGR YPKPLAELPG RTPIFWPGVM QSPPWRDARL ACTPHQGSIL LDKDGKRKHT RPTFSGQQIF ALEKTFEQTK YLAGPERARL AYSLGMTESQ VKVWFQNRRT KWRKKHAAEM ATAKKKQDSE TERLKGTSEN EEDDDDYNKP LDPNSDDEKI TQLLKKHKSS GGSLLLHASE AEGSS
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Required for the development of insulin-producing beta cells in the islets of Langerhans at the secondary transition. Involved in transcriptional regulation of the insulin gene. Together with NKX2-2 and IRX3, restricts the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals.
基因功能參考文獻(xiàn)：
1. our data reveal a novel mechanism of Notch1 transcriptional regulation in the ventral spinal cord by Nkx6.1 via its binding with Notch1 enhancer CR2 during embryonic development. PMID: 27924849
2. Nkx6.1 plays a vital role in astrocyte specification and differentiation in the ventral spinal cord. PMID: 25285789
3. Over time, Nkx6.1-deficient beta cells acquired molecular characteristics of delta cells, revealing a molecular link between impaired beta cell functional properties and loss of cell identity. PMID: 24035389
4. Our findings establish Nkx6.1 as a beta cell programming factor and demonstrate that repression of alternative lineage programs is a fundamental principle by which beta cells are specified and maintained. PMID: 23382704
5. Data found direct binding of RBP-jkappa to the Nkx6.1 proximal promoter. PMID: 22461559
6. Results demonstrate that sustained Nkx6.1 overexpression in vivo does not stimulate beta-cell proliferation, expand beta-cell mass, or improve glucose metabolism in either normal or beta-cell-depleted pancreata. PMID: 21964593
7. Nkx6 genes pattern the frog neural plate and Nkx6.1 is necessary for motoneuron axon projection. PMID: 21035438
8. Data show a longitudinal domain positive for both Nkx6.1 and Nkx6.2 that is medial to the Pou4f1-positive red nucleus. This domain could correspond to part of the reticular formation, which extends from the diencephalon and the mesencephalon. PMID: 20549744
9. Ependymal cells in adult spinal cord are derived from Nkx6.1+ ventral neural progenitor cells. Nkx6.1+ ependymal cells in adult mouse spinal cords may retain the proliferative property of neural stem cells. PMID: 12528188
10. Results demonstrate a requirement for Nkx6.1 in the development of postmitotic motoneurons, and suggest a cell-autonomous function in the control of branchio-motoneuron migration. PMID: 14534138
11. Nkx6.1 homeodomain neural progenitor gene is specifically expressed in the ventral neural tube, and its activity is required for motoneuron generation in the spinal cord. PMID: 14602683
12. Nkx6.1 is a bifunctional transcription factor that serves to maintain the specific expression of its own gene during beta-cell differentiation while simultaneously effecting broader gene repression events PMID: 15056733
13. Nkx6.1 is regulated by Nkx6.2; Nkx6.1 has distinct domains in the pancreas. PMID: 15944193
14. distinct requirements for Nkx6.1 and Nkx6.2 in endocrine differentiation are a consequence of their divergent spatiotemporal expression domains rather than their biochemical activities PMID: 17537793
15. Nkx6 proteins, a set of Hox-regulated homeodomain transcription factors, are expressed by motor pools soon after motor neurons leave the cell cycle, before the formation of muscle nerve side branches in the limb of mice. PMID: 18215620
16. confocal analysis identifies an abundant triple-positive (Ptf1a(+)/Nkx6.1(+)/Pdx1(+)) putative early multipotent pancreatic progenitor cell that marks the e9.5 dorsal pancreas and e10.5 ventral pancreas. PMID: 18347078
17. Data show that subtype-restricted progenitors from the Nkx6.1+ region are present in the ventral spinal cord, although at a lower frequency than expected. PMID: 19123929
18. The homeodomain transcription factor Nkx6-1 controls the proper development of the red nucleus and of the oculomotor and trochlear nucleus neurons. PMID: 19592574
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亞細(xì)胞定位：

Nucleus.
組織特異性：

Expressed by neuronal progenitor cells in discrete domains of the ventral neural tube. In the pancreas, expressed exclusively in insulin-producing beta cells of the islets of Langerhans (at protein level).
數(shù)據(jù)庫鏈接：

KEGG: mmu:18096

STRING: 10090.ENSMUSP00000042716

UniGene: Mm.193072