Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ1321 (MJ1321), partial

中文名稱：

賈納斯奇甲烷球菌MJ1321重組蛋白
貨號：

CSB-YP710424MRU
規(guī)格：
來源：

Yeast
其他：

中文名稱：

賈納斯奇甲烷球菌MJ1321重組蛋白
貨號：

CSB-EP710424MRU
規(guī)格：
來源：

E.coli
其他：

中文名稱：

賈納斯奇甲烷球菌MJ1321重組蛋白
貨號：

CSB-EP710424MRU-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

賈納斯奇甲烷球菌MJ1321重組蛋白
貨號：

CSB-BP710424MRU
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

賈納斯奇甲烷球菌MJ1321重組蛋白
貨號：

CSB-MP710424MRU
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

MJ1321
Uniprot No.：

Q58717
別名：

MJ1321; Protein argonaute; MjAgo; EC 3.1.25.-
種屬：

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
蛋白長度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

An endodeoxyribonuclease that may play a role in defense against invading genetic elements. Uses short DNA sequences as guides to bind complementary target strands, resulting in slicing of the target DNA. Also has guide-independent activity on target DNA. Probably a first round of guide-independent activity on an invading plasmid or virus would generate guide DNAs for subsequent, more efficient, guide-dependent degradation of invading nucleic acids. Endonucleolytically cleaves DNA in short dsDNA (the guide DNA indicates where to cleave on the target DNA). Efficient guide-dependent target DNA cleavage requires a minimal length of 15 bp and is maximal at 19 bp. Prefers guide DNA with 5'-phosphorylated purines and 3'-pyrimidines; changing these bases alters the cleavage activity and patterns. Has no activity on substrate with a mismatch at positions 10 and 11, ssDNA or RNA, nor on DNA-RNA hybrids. Digests longer (750 bp) dsDNA as well as circular plasmid and naked genomic DNA, but not chromatin, in a guide DNA-independent manner. Addition of endogenous histone A3 protects DNA from cleavage, while cleavage is insensitive to methylation. When plasmid encoding active or mutated protein (Ala-541) is transformed into Sulfolobus acidocaldarius about 25-fold fewer transformants are found with active protein; reduced levels of plasmid are found in wild-type transformed cells. While S.acidocaldarius grows at a similar temperature to M.jannaschii (70 to 80 degrees Celsius) it has very different histone-like proteins, which presumably do not protect against MjAgo. Binds ssDNA, dsDNA and DNA-RNA hybrids; binding is most efficient with dsDNA.
數(shù)據(jù)庫鏈接：

KEGG: mja:MJ_1321

STRING: 243232.MJ_1321