Recombinant Human Leucine-rich repeat and WD repeat-containing protein 1 (LRWD1)

中文名稱：

人LRWD1重組蛋白
貨號：

CSB-YP887017HU
規(guī)格：
來源：

Yeast
其他：

中文名稱：

人LRWD1重組蛋白
貨號：

CSB-EP887017HU
規(guī)格：
來源：

E.coli
其他：

中文名稱：

人LRWD1重組蛋白
貨號：

CSB-EP887017HU-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人LRWD1重組蛋白
貨號：

CSB-BP887017HU
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

人LRWD1重組蛋白
貨號：

CSB-MP887017HU
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

LRWD1
Uniprot No.：

Q9UFC0
別名：

1200011O22Rik; AU042569; AW548074; DKFZp434K1815; Leucine rich repeats and WD repeat domain containing 1; Leucine-rich repeat and WD repeat-containing protein 1; LRWD1; LRWD1_HUMAN; ORC-associated protein; ORCA; Origin recognition complex-associated protein
種屬：

Homo sapiens (Human)
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-647
氨基酸序列

MGPLSARLLM QRGRPKSDRL GKIRSLDLSG LELLSEHLDP KLLCRLTQLQ ELDLSNNHLE TLPDNLGLSH LRVLRCANNQ LGDVTALCQF PKLEELSLEG NPFLTVNDNL KVSFLLPTLR KVNGKDASST YSQVENLNRE LTSRVTAHWE KFMATLGPEE EAEKAQADFV KSAVRDVRYG PESLSEFTQW RVRMISEELV AASRTQVQKA NSPEKPPEAG AAHKPRARLA ALKRPDDVPL SLSPSKRACA SPSAQVEGSP VAGSDGSQPA VKLEPLHFLQ CHSKNNSPQD LETQLWACAF EPAWEEGATS QTVATCGGEA VCVIDCQTGI VLHKYKAPGE EFFSVAWTAL MVVTQAGHKK RWSVLAAAGL RGLVRLLHVR AGFCCGVIRA HKKAIATLCF SPAHETHLFT ASYDKRIILW DIGVPNQDYE FQASQLLTLD TTSIPLRLCP VASCPDARLL AGCEGGCCCW DVRLDQPQKR RVCEVEFVFS EGSEASGRRV DGLAFVNEDI VASKGSGLGT ICLWSWRQTW GGRGSQSTVA VVVLARLQWS STELAYFSLS ACPDKGIVLC GDEEGNVWLY DVSNILKQPP LLPAALQAPT QILKWPQPWA LGQVVTKTMV NTVVANASFT YLTALTDSNI VAIWGRM
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.
基因功能參考文獻(xiàn)：
1. Nuclear factor erythroid-2-related factor regulates LRWD1 expression and cellular adaptation to oxidative stress in human embryonal carcinoma cells PMID: 29544732
2. a novel function of LRWD1 in controlling microtubule nucleation and cell cycle progression in the human testicular embryonic carcinoma cells. PMID: 28569402
3. LRWD1 is a novel regulator of EWS-FLI1 driven cell proliferation in Ewing sarcoma cells. EWS-FLI1 regulates LRWD1 expression and LRWD1 may contribute to EWS-FLI1 mediated transcriptional repression. PMID: 27760381
4. Data show that ORCA acts as a scaffold for the establishment of H3K9 KMT complex. PMID: 25922909
5. This is the first study showing that the expression of the testis-enriched LRWD1 gene is regulated by NF-kappaB. PMID: 23275029
6. Data (from studies using recombinant proteins) suggest that deletion of leucine rich (LRR) domain significantly down-regulates expression of LRWD1 protein; LRR domain is significantly correlated with stability of LRWD1 protein. PMID: 25170821
7. three coding single-nucleotide polymorphisms (SNP1-SNP3) could be detected in Japanese patients with Sertoli cell-only syndrome PMID: 23445371
8. Orc2 protects ORCA from ubiquitin-mediated degradation in vivo. PMID: 22935713
9. propose that the dynamic association of ORCA with pre-RC components modulates the assembly of its interacting partners on chromatin and facilitates DNA replication initiation PMID: 22645314
10. Leucine-rich repeat and WD repeat-containing protein 1 is recruited to pericentric heterochromatin by trimethylated lysine 9 of histone H3 and maintains heterochromatin silencing PMID: 22427655
11. Results suggest ORCA-mediated association of ORC to chromatin is critical to initiate preRC assembly in G1 and chromatin organization in post-G1 cells. PMID: 20932478
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亞細(xì)胞定位：

Nucleus. Chromosome, centromere. Chromosome, telomere. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Note=Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres. Recruitment to pericentric heterochromatin largely depends on the presence of H3K9me3.
蛋白家族：

LRWD1 family
組織特異性：

Testis-specific. Drastically down-regulated in testis from patients with Sertoli cell-only syndrome (SCOS).
數(shù)據(jù)庫鏈接：

HGNC: 21769

OMIM: 615167

KEGG: hsa:222229

STRING: 9606.ENSP00000292616

UniGene: Hs.274135