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  4. Recombinant Human DNA oxidative demethylase ALKBH2 (ALKBH2)

Recombinant Human DNA oxidative demethylase ALKBH2 (ALKBH2)

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  • 中文名稱：
    Recombinant Human DNA oxidative demethylase ALKBH2 (ALKBH2)
  • 品名簡(jiǎn)稱：
    Recombinant Human ALKBH2 protein
  • 貨號(hào)：
    CSB-EP761219HUf0
  • 說(shuō)明書：
  • 規(guī)格：
    ￥1344
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    ALKBH2
  • Uniprot No.：
  • 別名：
    Alkylated DNA repair protein alkB homolog 2;Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2;Oxy DC1
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Full Length
  • 來(lái)源：
    E.coli
  • 分子量：
    60.9 kDa
  • 表達(dá)區(qū)域：
    1-261aa
  • 氨基酸序列
    MDRFLVKGAQGGLLRKQEEQEPTGEEPAVLGGDKESTRKRPRREAPGNGGHSAGPSWRHIRAEGLDCSYTVLFGKAEADEIFQELEKEVEYFTGALARVQVFGKWHSVPRKQATYGDAGLTYTFSGLTLSPKPWIPVLERIRDHVSGVTGQTFNFVLINRYKDGCDHIGEHRDDERELAPGSPIASVSFGACRDFVFRHKDSRGKSPSRRVAVVRLPLAHGSLLMMNHPTNTHWYHSLPVRKKVLAPRVNLTFRKILLTKK
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    C-terminal GST-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
  • 基因功能參考文獻(xiàn)：
    1. These results revealed that N3-ethylthymidine , but not other DNA lesions, could be repaired by Alkbh2 and Alkbh3 in mammalian cells. PMID: 26930515
    2. studies reveal the ALKBH2 binding interface of PCNA and indicate that both germline and somatic ALKBH2 variants could have cellular effects on ALKBH2 function in DNA repair. PMID: 26408825
    3. It was shown for first time that DNA glycosylase ALKBH2 can repair DNA adduct 1,N2-ethenoguanine. PMID: 25797601
    4. ABH2 knockdown impairs rDNA transcription and leads to increased single-stranded and double-stranded DNA breaks in the rDNA genes. PMID: 23972994
    5. Overexpression of ALKBH2 is associated with enhanced resistance to temozolomide in glioblastoma. PMID: 23258843
    6. ALKBH2 is an upstream molecule of the oncoprotein, MUC1, and regulates cell cycle and EMT, resulting in progression of urothelial carcinomas. PMID: 23279696
    7. combination of duplex interrogation and oxidation chemistry allows ALKBH2 to detect and process diverse lesions efficiently and correctly PMID: 22659876
    8. The non-enzymatic binding of AAG to 3,N(4)-ethenocytosine specifically blocks ALKBH2-catalyzed repair of 3,N(4)-ethenocytosine but not that of methylated ALKBH2 substrates. PMID: 22079122
    9. X-ray absorption spectroscopy structural investigation of early intermediates in the mechanism of DNA repair by human ABH2 PMID: 21510633
    10. ABH2 is downregulated in a subset of gastric cancers, and might be involved in the molecular mechanism of gastric cancer through inhibiting the proliferation of gastric cancer cells. PMID: 21155885
    11. This work has provided a detailed understanding of the structural features of the single-stranded DNA and double-stranded DNA preferences of ABH2 and ABH3. PMID: 20714506
    12. Divergent sequences outside of the active site determine substrate specificities of ABH2. PMID: 20525795
    13. Tumour protein (TP53) is directly involved by binding to the promoter of ALKBH2 in mediating photofrin-mediaated photodynamic therapy in U87 glioma cells. PMID: 20661249
    14. first crystal structure of ABH2 PMID: 18432238
    15. ABH2 is active in the direct reversal of epsilon A lesions, and that ABH2, together with the alkyl-N-adenine-DNA glycosylase, which is the most effective enzyme for the repair of epsilon A, comprise the cellular defense against epsilon A lesions PMID: 18519673
    16. Mutations of ABH2 is associated with pediatric brain tumors. PMID: 19290481

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  • 亞細(xì)胞定位：
    Nucleus. Note=Detected in replication foci during S-phase.
  • 蛋白家族：
    AlkB family
  • 組織特異性：
    Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 32487

    OMIM: 610602

    KEGG: hsa:121642

    STRING: 9606.ENSP00000343021

    UniGene: Hs.374458



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