Recombinant Drosophila melanogaster tRNA (guanine-N (7)-)-methyltransferase subunit wuho (wuho)

中文名稱：

黑腹果蠅wuho重組蛋白
貨號(hào)：

CSB-YP892937DLU
規(guī)格：
來源：

Yeast
其他：

中文名稱：

黑腹果蠅wuho重組蛋白
貨號(hào)：

CSB-EP892937DLU
規(guī)格：
來源：

E.coli
其他：

中文名稱：

黑腹果蠅wuho重組蛋白
貨號(hào)：

CSB-EP892937DLU-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

黑腹果蠅wuho重組蛋白
貨號(hào)：

CSB-BP892937DLU
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

黑腹果蠅wuho重組蛋白
貨號(hào)：

CSB-MP892937DLU
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

wuho
Uniprot No.：

Q9W415
別名：

wuho; CG15897; tRNA; guanine-N(7)-)-methyltransferase non-catalytic subunit wuho
種屬：

Drosophila melanogaster (Fruit fly)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-424
氨基酸序列

MCTTISFAEP EIVLGHGRRV LFVNPDDLQI FKEIELPPDL GLKGHTSQSQ ESCTAAAAAS TATAASGQAP GGKEQQLANQ PEEGGTSASA SGLGCATSTS VQNVAYSPDG QLLAVTTSGK QKALLLYRSR PENARLLSAR PLARASSALR FCSDSSSILV TDKTGDCYQY DCVEVEAPPR LLLGHLSVVY DILWSEDQQH IITCDRDDKI RVTNYPATFD IHSYCLGHRE FVSGLALLTE QHIASASGDK TLRVWNYIQG KELLQHELPA PAVRLLVRQL EPEKVFQAAV LFYEHVDALG LYRLERSSDD TWSVTATQLV CAEAGSWSIS NFTLTSDRIY ITGAENERLS LRVYDIATGQ PASSGVPEGW LKMVLDGLGA NEEGAPPFIP EDLSVWFKKR FDNVSDYLER KKRRIEEQQQ QKCG
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit. Required during gametogenesis.
基因功能參考文獻(xiàn)：
1. CG15897, here named wuho (wh) contains 5 WD40 repeats; has a critical function in cellular differentiation for germline cells during gametogenesis PMID: 16762337
亞細(xì)胞定位：

Nucleus. Note=Localized to the nuclei of nurse cells, follicle cells and oocytes at early stages, from germarium to stage 4 egg chambers. Also present in the nuclei of spermatocytes and in the apical cells of the testes.
蛋白家族：

WD repeat TRM82 family
組織特異性：

In testis, it is present at high level in hub cells, a niche for germline stem cells of testis.
數(shù)據(jù)庫鏈接：

KEGG: dme:Dmel_CG15897

STRING: 7227.FBpp0070862

UniGene: Dm.31038