Recombinant Drosophila melanogaster Protein-cysteine N-palmitoyltransferase Rasp (rasp), partial

中文名稱：

黑腹果蠅rasp重組蛋白
貨號：

CSB-YP893826DLU
規格：
來源：

Yeast
其他：

中文名稱：

黑腹果蠅rasp重組蛋白
貨號：

CSB-EP893826DLU
規格：
來源：

E.coli
其他：

中文名稱：

黑腹果蠅rasp重組蛋白
貨號：

CSB-EP893826DLU-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

黑腹果蠅rasp重組蛋白
貨號：

CSB-BP893826DLU
規格：
來源：

Baculovirus
其他：

中文名稱：

黑腹果蠅rasp重組蛋白
貨號：

CSB-MP893826DLU
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

rasp
Uniprot No.：

Q9VZU2
別名：

rasp; cmn; sit; ski; CG11495; Protein-cysteine N-palmitoyltransferase Rasp; EC 2.3.1.-; Protein central missing; Protein sightless; Protein skinny hedgehog
種屬：

Drosophila melanogaster (Fruit fly)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Required in hedgehog (hh) expressing cells for production of appropriate signaling activity in embryos and in the imaginal precursors of adult tissues. Acts within the secretory pathway to catalyze N-terminal palmitoylation of Hh; this lipid modification is required for the embryonic and larval patterning activities of the Hh signal. Not required for Wg signaling.
基因功能參考文獻：
1. These findings define residues and regions within Shh that are necessary for its recognition as a substrate for Hhat-mediated palmitoylation. PMID: 23112049
2. Rasp-dependent acylation is necessary to generate a fully active Hh protein. PMID: 11861468
3. Rasp promotes palmitate addition to the N-terminal cysteine residue of Spitz, and this cysteine is required for Spitz activity in vivo PMID: 16459296
亞細胞定位：

Membrane; Multi-pass membrane protein.
蛋白家族：

Membrane-bound acyltransferase family, HHAT subfamily
數據庫鏈接：

KEGG: dme:Dmel_CG11495

STRING: 7227.FBpp0072893

UniGene: Dm.2681