Recombinant Drosophila melanogaster E3 ubiquitin-protein ligase Smurf1 (lack), partial

中文名稱：

黑腹果蠅lack重組蛋白
貨號：

CSB-YP895578DLU
規格：
來源：

Yeast
其他：

中文名稱：

黑腹果蠅lack重組蛋白
貨號：

CSB-EP895578DLU
規格：
來源：

E.coli
其他：

中文名稱：

黑腹果蠅lack重組蛋白
貨號：

CSB-EP895578DLU-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

黑腹果蠅lack重組蛋白
貨號：

CSB-BP895578DLU
規格：
來源：

Baculovirus
其他：

中文名稱：

黑腹果蠅lack重組蛋白
貨號：

CSB-MP895578DLU
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

lack
Uniprot No.：

Q9V853
別名：

Smurf; lack; Smurf1; CG4943; E3 ubiquitin-protein ligase Smurf1; EC 2.3.2.26; HECT-type E3 ubiquitin transferase Smurf1; Lethal with a checkpoint kinase protein; SMAD ubiquitination regulatory factor 1 homolog; DSmurf
種屬：

Drosophila melanogaster (Fruit fly)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Down-regulates Dpp signaling after gastrulation by promoting MAD ubiquitination and subsequent degradation.
基因功能參考文獻：
1. dSmurf is required for synapse remodeling and axon pruning by glia PMID: 28057437
2. Interaction with Slimb leads to the ubiquitination and degradation of dSmurf, resulting in negative regulation of dSmurf-mediated Yki phosphorylation and activity in the Hippo pathway. Thus our study revealed a new regulatory mechanism of the Hippo pathway which may provide implications for developing tumor treatment. PMID: 27856247
3. Sufficient knock-down of dSmurf increases the protein abundance of Wts and thus increases phosphorylation level at S168 of Yki, the key downstream target of Wts in the Hippo pathway. PMID: 25450375
4. Smo interacts with Smurf and promotes it to mediate Patched ubiquitination by targeting the K1261 site in Ptc. PMID: 24302888
5. Smurf-mediated differential proteolysis generates dynamic BMP signaling in germline stem cells during Drosophila testis development. PMID: 23988579
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數據庫鏈接：

KEGG: dme:Dmel_CG4943

STRING: 7227.FBpp0305371

UniGene: Dm.2783