Recombinant Cupriavidus necator Queuine tRNA-ribosyltransferase (tgt)

中文名稱：

自體連接器tgt重組蛋白
貨號(hào)：

CSB-YP610299DZQ
規(guī)格：
來源：

Yeast
其他：

中文名稱：

自體連接器tgt重組蛋白
貨號(hào)：

CSB-EP610299DZQ
規(guī)格：
來源：

E.coli
其他：

中文名稱：

自體連接器tgt重組蛋白
貨號(hào)：

CSB-EP610299DZQ-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

自體連接器tgt重組蛋白
貨號(hào)：

CSB-BP610299DZQ
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

自體連接器tgt重組蛋白
貨號(hào)：

CSB-MP610299DZQ
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

tgt
Uniprot No.：

Q0K733
別名：

tgt; H16_A3113; Queuine tRNA-ribosyltransferase; EC 2.4.2.29; Guanine insertion enzyme; tRNA-guanine transglycosylase
種屬：

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-376
氨基酸序列

MLNFELITTD GNARRGRVTL NHGVVETPIF MPVGTYGSVK AMSPLELNEI GAHIILGNTF HLWLRPGLDV VNAHEGLHKF IGWDKPILTD SGGFQVFSLG DLRKITEDGV TFASPVNGDK LFLSPEISMQ IQRTLNSDIV MQFDECTPYE IDGRAATHEE AAKSMRMSLR WAKRSRDEFE RLANPNALFG IVQGGMYEDL RDESLAGLSE LDFHGFAIGG LSVGEPKEDM MRVLEHVAPR LPAHKPHYLM GVGTPEDLVA GVAAGVDMFD CVMPTRNARN GWLFTRFGDV KIKNAAHRND PRPLDESCAC YTCRNFSRAY LHHLHRVGEI LGARLNTIHN LHYYLQLMRE MREAIEHHRF ADFRRQFAAD RARGTQ
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
蛋白家族：

Queuine tRNA-ribosyltransferase family
數(shù)據(jù)庫鏈接：

KEGG: reh:H16_A3113

STRING: 381666.H16_A3113