Recombinant Claviceps fusiformis Tryptophan dimethylallyltransferase (dmaW)

中文名稱：

Recombinant Claviceps fusiformis Tryptophan dimethylallyltransferase(dmaW)
貨號(hào)：

CSB-YP619619CBAM
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Claviceps fusiformis Tryptophan dimethylallyltransferase(dmaW)
貨號(hào)：

CSB-EP619619CBAM
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Claviceps fusiformis Tryptophan dimethylallyltransferase(dmaW)
貨號(hào)：

CSB-EP619619CBAM-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Claviceps fusiformis Tryptophan dimethylallyltransferase(dmaW)
貨號(hào)：

CSB-BP619619CBAM
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Claviceps fusiformis Tryptophan dimethylallyltransferase(dmaW)
貨號(hào)：

CSB-MP619619CBAM
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

dmaW
Uniprot No.：

Q12594
別名：

dmaWTryptophan dimethylallyltransferase; EC 2.5.1.34; 4-dimethylallyltryptophan synthase; DMATS; All-trans-hexaprenyl-diphosphate synthase; L-tryptophan dimethylallyl transferase
種屬：

Claviceps fusiformis (Ergot fungus)
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-455
氨基酸序列

MMTKAPATAV YDTLSLLFDF PNQEQRLWWH SIAPMFAAML DTAGHNVHDQ YRHLGIFKKH IIPFLGVYPA QGKHTWPSVL TRYGIPFELS LNCLDSVVRY TFEPTTEHTG TGDDSYNAFA ILECIQKLVR IQPGIDMEWF SYFRNELVLN ATESARLGRN DSVNQQPIRT QNKLALDLKG DRFALKVYLY PHLKSIATGV SSHDLIFNSV RKLSQKHTSI QPSFNVLCDY VASRNDPDSN AAEAEAGVPA SALRARLLSC DLVDPSKSRI KIYLLEQTVS LTAMEDLWTL GGRRTDSSTL NGLDMMRELW HLLQIPSGFM KYPESDLKLG EVPDEQLPSM VHYALHPDQP MPEPQVYFTV FGMSDAGITN ALATFFSRHG WYEMAKKYRV FLEGSFPNHD FESLNYLHTY VSFSYRKNKP YLSVYLHSFE TGQWPAFSDD PTAFNAFKRC DLSLT
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Tryptophan dimethylallyltransferase; part of the gene cluster that mediates the biosynthesis of fungal ergot alkaloid. DmaW catalyzes the first step of ergot alkaloid biosynthesis by condensing dimethylallyl diphosphate (DMAP) and tryptophan to form 4-dimethylallyl-L-tryptophan. The second step is catalyzed by the methyltransferase easF that methylates 4-dimethylallyl-L-tryptophan in the presence of S-adenosyl-L-methionine, resulting in the formation of 4-dimethylallyl-L-abrine. The catalase easC and the FAD-dependent oxidoreductase easE then transform 4-dimethylallyl-L-abrine to chanoclavine-I which is further oxidized by easD in the presence of NAD(+), resulting in the formation of chanoclavine-I aldehyde. Agroclavine dehydrogenase easG then mediates the conversion of chanoclavine-I aldehyde to agroclavine via a non-enzymatic adduct reaction: the substrate is an iminium intermediate that is formed spontaneously from chanoclavine-I aldehyde in the presence of glutathione. Further conversion of agroclavine to paspalic acid is a two-step process involving oxidation of agroclavine to elymoclavine and of elymoclavine to paspalic acid, the second step being performed by the elymoclavine oxidase cloA. However, cloA does not encode a functional enzyme indicating that C.fusiformis terminates its ergot alkaloid pathway at elymoclavine.
蛋白家族：

Tryptophan dimethylallyltransferase family