Recombinant Bovine NAD-dependent protein deacetylase sirtuin-4 (SIRT4)

中文名稱：

牛SIRT4重組蛋白
貨號：

CSB-YP628201BO
規(guī)格：
來源：

Yeast
其他：

中文名稱：

牛SIRT4重組蛋白
貨號：

CSB-EP628201BO
規(guī)格：
來源：

E.coli
其他：

中文名稱：

牛SIRT4重組蛋白
貨號：

CSB-EP628201BO-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

牛SIRT4重組蛋白
貨號：

CSB-BP628201BO
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

牛SIRT4重組蛋白
貨號：

CSB-MP628201BO
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

SIRT4
Uniprot No.：

Q1JQC6
別名：

SIRT4; NAD-dependent protein lipoamidase sirtuin-4; mitochondrial; EC 2.3.1.-; NAD-dependent ADP-ribosyltransferase sirtuin-4; EC 2.4.2.-; NAD-dependent protein deacetylase sirtuin-4; EC 2.3.1.286; Regulatory protein SIR2 homolog 4; SIR2-like protein 4
種屬：

Bos taurus (Bovine)
蛋白長度：

Full Length of Mature Protein
表達區(qū)域：

30-315
氨基酸序列

T GLFVPPSPPL DPEKVKELQR FITLSKRLLV MTGAGISTES GIPDYRSEKV GLYARTDRRP IQHGDFVRSA PVRQRYWARN FVGWPQFSSR QPNPAHWALS NWERLGKLHW LVTQNVDALH TKAGSQRLTE LHGCMHRVLC LDCGEQTPRG VLQERFQVLN PTWSAEAHGL APDGDVFLTE EEVQSFQVPS CSRCGGPLKP DVVFFGDTVK PDKVDFVHKR VKEADSLLVV GSSLQVYSGY RFILTAREKK LPIVILNIGP TRSDDLASLK LDSRCGELLP LIDPR
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor. Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis. Does not seem to deacetylate PC. Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably through the regulation of NAD(+) levels. Down-regulates insulin secretion.
亞細胞定位：

Mitochondrion matrix.
蛋白家族：

Sirtuin family, Class II subfamily
數(shù)據(jù)庫鏈接：

KEGG: bta:519328

STRING: 9913.ENSBTAP00000028210

UniGene: Bt.39710