Recombinant Arabidopsis thaliana Adagio protein 3 (ADO3), partial

中文名稱：

Recombinant Arabidopsis thaliana Adagio protein 3(ADO3) ,partial
貨號：

CSB-YP883722DOA
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Arabidopsis thaliana Adagio protein 3(ADO3) ,partial
貨號：

CSB-EP883722DOA
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Arabidopsis thaliana Adagio protein 3(ADO3) ,partial
貨號：

CSB-EP883722DOA-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Arabidopsis thaliana Adagio protein 3(ADO3) ,partial
貨號：

CSB-BP883722DOA
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Arabidopsis thaliana Adagio protein 3(ADO3) ,partial
貨號：

CSB-MP883722DOA
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

ADO3
Uniprot No.：

Q9C9W9
別名：

ADO3; FKF1; At1g68050; T23K23.10Adagio protein 3; F-box only protein 2a; FBX2a; Flavin-binding kelch repeat F-box protein 1
種屬：

Arabidopsis thaliana (Mouse-ear cress)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Component of an E3 ubiquitin ligase complex that plays a central role in blue light-dependent circadian cycles. Acts as a blue light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO3) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including transition to flowering time, hypocotyl elongation, cotyledons and leaf movement rhythms. Forms a complex with 'GIGANTEA' (GI) to regulate 'CONSTANS' (CO) expression. Promotes CO expression during the light period of long days by decreasing the stability of CDF1 and CDF2 and by interacting directly with the CO protein and stabilizing it. ADO3 function is mainly GI dependent. Does not act as a regulator of CDF1 transcription. The interactions of ADO1/ZTL and ADO2 with ADO3 prevent its interaction with CDF1.
基因功能參考文獻：
1. FKF1 inhibits CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1)-dependent CO degradation. Light-activated FKF1 has no apparent effect on COP1 stability but can interact with and negatively regulate COP1. We show that FKF1 can inhibit COP1 homo-dimerization. Mutation of the coiled-coil domain in COP1, which prevents dimer formation, impairs COP1 function in coordinating flowering time. PMID: 29273730
2. study demonstrates that FKF1 protein stabilizes CONSTANS (CO)protein in the afternoon in long days; together with CO transcriptional regulation, FKF1 protein controls robust FLOWERING LOCUS T FT) mRNA induction through multiple feedforward mechanisms that accurately control flowering timing PMID: 22628657
3. This result indicates that the conformational change in the loop region represents a major change in the FKF1- light-oxygen-voltage domain photoreaction. PMID: 21112308
4. Quaternary structure of FKF1 protein PMID: 15710392
5. Photoreaction of the FKF1-LOV(LOV-voltage sensing domain binding a flavin mononucleotide)polypeptide was studied by low-temperature absorption spectroscopy PMID: 16953568
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亞細胞定位：

Nucleus. Cytoplasm. Note=Moves from the nucleus into cytosolic speckles upon interaction with ADO1 and ADO2.
蛋白家族：

ADAGIO family
組織特異性：

Highly expressed in stomata and leaves and to a lower extent in seeds, roots, rosettes, stems and siliques. Also present in sepals and anther filaments.
數據庫鏈接：

KEGG: ath:AT1G68050

STRING: 3702.AT1G68050.1

UniGene: At.20568