Recombinant Arabidopsis thaliana 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic (NCED3), partial

中文名稱：

擬南芥NCED3重組蛋白
貨號(hào)：

CSB-YP868001DOA
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

擬南芥NCED3重組蛋白
貨號(hào)：

CSB-EP868001DOA
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

擬南芥NCED3重組蛋白
貨號(hào)：

CSB-EP868001DOA-B
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

擬南芥NCED3重組蛋白
貨號(hào)：

CSB-BP868001DOA
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

擬南芥NCED3重組蛋白
貨號(hào)：

CSB-MP868001DOA
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

NCED3
Uniprot No.：

Q9LRR7
別名：

NCED3; STO1; At3g14440; MOA2.4; 9-cis-epoxycarotenoid dioxygenase NCED3; chloroplastic; AtNCED3; EC 1.13.11.51; Protein SALT TOLERANT 1
種屬：

Arabidopsis thaliana (Mouse-ear cress)
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids, in response to water stress.
基因功能參考文獻(xiàn)：
1. In wild-type leaves, methyl jasmonate (MeJA)stimulates the expression of NCED3, which encodes an enzyme in abscisic acid (ABA) biosynthesis. Endogenous ABA could be involved in MeJA signal transduction and lead to stomatal closure in the guard cells. PMID: 21402795
2. Overexpression of AtSTO1 improved salt tolerance in transgenic poplar. PMID: 24929937
3. A distal ABA responsive element in AtNCED3 promoter is required for positive feedback regulation of ABA biosynthesis in Arabidopsis. PMID: 24475264
4. An overlapping G-box recognition sequence (50-CACGTG-30) at 22248 base pairs from the transcriptional start site of AtNCED3 is an important cis-acting element in the induction of the dehydration response. PMID: 23604098
5. The ced1 (for 9-cis epoxycarotenoid dioxygenase defective 1) mutant isolated in this study showed markedly reduced expression of NCED3 in response to osmotic stress (polyethylene glycol) treatments compared with the wild type. PMID: 21610183
6. NCED5 thus contributes, together with NCED3, to abscisic acid production affecting plant growth and water stress tolerance. PMID: 22171989
7. ATX1 (histone-lysine N-methyltransferase) directly binds NCED3 and regulates its transcription. PMID: 21309869
8. Studies indicate that NCED3 encodes 9-cis-epoxycarotenoid dioxygenase that catalyzes the oxidative cleavage of a 9-cis isomer of epoxycarotenoid (9-cis-violaxanthin or 9'-cis-neoxanthin) to form xanthoxin. PMID: 21103336
9. The spatial constraint of AtNCED3 expression in vascular tissues provides a novel insight into plant systemic response to drought stresses. PMID: 18550687
10. Mutations in the SIS7/NCED3/STO1 gene confer a sugar-insensitive phenotype, which indicates that a role in ABA biosynthesis is not necessary to confer sugar insensitivity. PMID: 18854047
11. The 274th site (P<-->S) and the 327th site (P<-->R) amino acid variations might be the cause of abscisic acid content increase of 163av accession under drought stress. PMID: 19200156
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亞細(xì)胞定位：

Plastid, chloroplast stroma. Note=Partially bound to the thylakoid.
蛋白家族：

Carotenoid oxygenase family
組織特異性：

Localized in roots, leaves, stems, empty silique envelopes and seeds. Expressed at the point of organ attachment and the abscission zones in the plant.
數(shù)據(jù)庫(kù)鏈接：

KEGG: ath:AT3G14440

STRING: 3702.AT3G14440.1

UniGene: At.26346