Recombinant Saccharomyces cerevisiae Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 (STT3), partial

中文名稱：

釀酒酵母STT3重組蛋白
貨號：

CSB-YP334505SVG
規(guī)格：
來源：

Yeast
其他：

中文名稱：

釀酒酵母STT3重組蛋白
貨號：

CSB-EP334505SVG
規(guī)格：
來源：

E.coli
其他：

中文名稱：

釀酒酵母STT3重組蛋白
貨號：

CSB-EP334505SVG-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

釀酒酵母STT3重組蛋白
貨號：

CSB-BP334505SVG
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

釀酒酵母STT3重組蛋白
貨號：

CSB-MP334505SVG
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

STT3
Uniprot No.：

P39007
別名：

STT3; YGL022WDolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3; Oligosaccharyl transferase subunit STT3; EC 2.4.99.18; Staurosporine and temperature sensitivity protein 3
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. This subunit contains the active site and the acceptor peptide and donor lipid-linked oligosaccharide (LLO) binding pockets.
基因功能參考文獻：
1. results show that most of the STT3 TMDs are well inserted into the ER membrane on their own or in the presence of the natural flanking residues. PMID: 28512128
2. Eukaryotic N-glycosylation occurs via the membrane-anchored C-terminal domain of the Stt3p subunit of oligosaccharyltransferase. PMID: 22865878
3. The cloning, overexpression, purification, and biophysical characterization of the carboxyl-terminal domain of Stt3p is reported; Asp(518) plays a critical structural role, in addition to its previously proposed catalytic role. PMID: 20047336
4. is an essential player in N-glycosylation PMID: 15590627
5. results suggest that membrane protein STT3 has 11 transmembrane helices and an overall N(cyt)-C(lum) orientation PMID: 15781470
6. Homologous Stt3 proteins can serve analogous functions in distantly related eukaryotic cells regardless of their degree of conservation. PMID: 15936094
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亞細(xì)胞定位：

Endoplasmic reticulum membrane; Multi-pass membrane protein.
蛋白家族：

STT3 family
數(shù)據(jù)庫鏈接：

KEGG: sce:YGL022W

STRING: 4932.YGL022W