Acetyl-Histone H2B type 1-B (K20) Recombinant Monoclonal Antibody
產品詳情
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產品描述：
CSB-RA010402A20acHU是針對組蛋白H2B第20位賴氨酸乙酰化修飾（Acetyl-Histone H2B type 1-B K20）開發的重組單克隆抗體。組蛋白H2B作為核小體核心成分，其K20位點的乙酰化修飾在表觀遺傳調控中發揮重要作用，參與染色質結構重塑、轉錄激活及DNA損傷修復等生物學過程。本產品經過ELISA、Western Blot（WB）、免疫細胞化學（ICC）、免疫熒光（IF）及流式細胞術（FC）等多平臺驗證，在WB實驗中表現出高特異性，推薦使用1:5000-1:10000稀釋比例可清晰檢測目標條帶；ICC/IF應用推薦1:50-1:500及1:30-1:200稀釋范圍，適用于細胞定位分析，能精準顯示核內乙酰化修飾信號；FC檢測則支持細胞群體表觀遺傳狀態分析。該抗體適用于染色質動態研究、組蛋白翻譯后修飾機制探索，以及發育生物學、腫瘤微環境等領域中表觀遺傳調控網絡的科研工作，為探索基因表達調控、干細胞多能性維持及疾病相關信號通路提供可靠工具。
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Uniprot No.：
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基因名：
HIST1H2BB
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別名：
HIST1H2BB antibody; H2BFFHistone H2B type 1-B antibody; Histone H2B.1 antibody; Histone H2B.f antibody; H2B/f antibody
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反應種屬：
Human
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免疫原：
A synthesized peptide
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免疫原種屬：
Homo sapiens (Human)
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標記方式：
Non-conjugated
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克隆類型：
Monoclonal
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抗體亞型：
Rabbit IgG
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純化方式：
Affinity-chromatography
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克隆號：
21F11
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濃度：
It differs from different batches. Please contact us to confirm it.
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保存緩沖液：
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：
Liquid
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應用范圍：
ELISA, WB, ICC, IF, FC
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推薦稀釋比：
| Application |
Recommended Dilution |
| WB |
1:5000-1:10000 |
| ICC |
1:50-1:500 |
| IF |
1:30-1:200 |
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Protocols：
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儲存條件：
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
靶點詳情
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功能：
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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基因功能參考文獻：
- Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants has been uncovered. PMID: 29643390
- RNF20 and H2Bub1 promotes chronic colonic inflammation and inflammation-associated colorectal cancer in mice and humans, partly by augmenting NF-kappaB activity and attenuating the antitumoral T cell response. PMID: 26854224
- RNF20-mediated H2B ubiquitination at DSBs plays a critical role in HRR through chromatin remodeling PMID: 21362548
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亞細胞定位：
Nucleus. Chromosome.
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蛋白家族：
Histone H2B family
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數據庫鏈接：
Most popular with customers
Western Blot
Immunocytochemistry analysis of CSB-RA010402A20acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010402A20acHU at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay histogram showing Hela cells stained with CSB-RA010402A20acHU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
